Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4218054..4218685 Replicon chromosome
Accession NZ_CP015228
Organism Escherichia coli strain 09-00049

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag GJ12_RS22930 Protein ID WP_001259388.1
Coordinates 4218410..4218685 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GJ12_RS22925 Protein ID WP_000593555.1
Coordinates 4218054..4218413 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ12_RS22900 4214186..4215130 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
GJ12_RS22905 4215127..4215960 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
GJ12_RS22910 4215960..4216724 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
GJ12_RS22915 4216721..4217527 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
GJ12_RS22920 4217533..4217934 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
GJ12_RS22925 4218054..4218413 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GJ12_RS22930 4218410..4218685 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
GJ12_RS22935 4218758..4219882 - 1125 WP_065225698.1 ABC transporter permease -
GJ12_RS22940 4219882..4222617 - 2736 WP_033817337.1 ribosome-associated ATPase/putative transporter RbbA -
GJ12_RS22945 4222614..4223681 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T62665 WP_001259388.1 NZ_CP015228:c4218685-4218410 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T62665 NZ_CP015228:c4218685-4218410 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT62665 WP_000593555.1 NZ_CP015228:c4218413-4218054 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT62665 NZ_CP015228:c4218413-4218054 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References