Detailed information of TA system TA062662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3188622..3189247
• Replicon: chromosome
• Accession: NZ_CP015228
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GJ12_RS17735
• Protein ID: WP_000911330.1
• Coordinates: 3188622..3189020 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GJ12_RS17740
• Protein ID: WP_000450524.1
• Coordinates: 3189020..3189247 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS17715	3184500..3184700	+	201	WP_000383836.1	YpfN family protein	-
GJ12_RS17720	3184810..3185508	-	699	WP_000679823.1	esterase	-
GJ12_RS17725	3185582..3187597	-	2016	WP_032197281.1	tRNA cytosine(34) acetyltransferase TmcA	-
GJ12_RS17730	3187612..3188475	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
GJ12_RS17735	3188622..3189020	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GJ12_RS17740	3189020..3189247	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GJ12_RS17745	3189401..3190114	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GJ12_RS17755	3190327..3191361	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
GJ12_RS17760	3191378..3192256	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GJ12_RS17765	3192402..3192974	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GJ12_RS17770	3192974..3193444	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T62662 WP_000911330.1 NZ_CP015228:c3189020-3188622 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T62662 NZ_CP015228:c3189020-3188622 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT62662 WP_000450524.1 NZ_CP015228:c3189247-3189020 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT62662 NZ_CP015228:c3189247-3189020 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33