Detailed information of TA system TA062653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2010038..2010564
• Replicon: chromosome
• Accession: NZ_CP015228
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: GJ12_RS11555
• Protein ID: WP_000323025.1
• Coordinates: 2010038..2010325 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: GJ12_RS11560
• Protein ID: WP_000534858.1
• Coordinates: 2010325..2010564 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS31290	2005715..2005846	-	132	WP_047626566.1	DUF3927 family protein	-
GJ12_RS11525	2006742..2007563	-	822	WP_000762885.1	antitermination protein	-
GJ12_RS11530	2007578..2007934	-	357	WP_000904111.1	RusA family crossover junction endodeoxyribonuclease	-
GJ12_RS11535	2007947..2008996	-	1050	WP_053292618.1	DUF968 domain-containing protein	-
GJ12_RS11540	2008998..2009276	-	279	WP_012304870.1	hypothetical protein	-
GJ12_RS11545	2009343..2009594	-	252	WP_000980994.1	hypothetical protein	-
GJ12_RS11550	2009811..2009966	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
GJ12_RS11555	2010038..2010325	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
GJ12_RS11560	2010325..2010564	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
GJ12_RS29115	2010589..2010894	+	306	WP_071527819.1	hypothetical protein	-
GJ12_RS11565	2011097..2011429	+	333	WP_001317460.1	FlxA-like family protein	-
GJ12_RS11570	2011866..2013206	-	1341	WP_000589012.1	ISNCY family transposase	-
GJ12_RS11575	2013240..2013659	-	420	WP_001151195.1	DUF977 family protein	-
GJ12_RS11580	2013700..2014665	-	966	WP_000054504.1	hypothetical protein	-
GJ12_RS11585	2014646..2015167	-	522	WP_000705349.1	hypothetical protein	-
GJ12_RS11590	2015151..2015378	-	228	WP_000476993.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1976333..2033611	57278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T62653 WP_000323025.1 NZ_CP015228:c2010325-2010038 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T62653 NZ_CP015228:c2010325-2010038 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT62653 WP_000534858.1 NZ_CP015228:c2010564-2010325 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT62653 NZ_CP015228:c2010564-2010325 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8