Detailed information of TA system TA062639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 2705328..2706125
• Replicon: chromosome
• Accession: NZ_CP015227
• Organism: Enterobacter sp. ODB01

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: A4308_RS12825
• Protein ID: WP_176450254.1
• Coordinates: 2705601..2706125 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A2W0G3H3
• Locus tag: A4308_RS12820
• Protein ID: WP_014883303.1
• Coordinates: 2705328..2705597 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4308_RS12790	2700949..2701449	+	501	WP_014883299.1	DedA family protein	-
A4308_RS12795	2701553..2702794	+	1242	WP_023337066.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
A4308_RS12800	2702828..2703055	-	228	WP_014883300.1	YccJ family protein	-
A4308_RS12805	2703074..2703670	-	597	WP_014883301.1	NAD(P)H:quinone oxidoreductase	-
A4308_RS12810	2704062..2704232	+	171	WP_023332656.1	general stress protein	-
A4308_RS12815	2704349..2705266	+	918	WP_047028038.1	DMT family transporter	-
A4308_RS12820	2705328..2705597	+	270	WP_014883303.1	DUF1778 domain-containing protein	Antitoxin
A4308_RS12825	2705601..2706125	+	525	WP_176450254.1	GNAT family N-acetyltransferase	Toxin
A4308_RS12830	2706199..2707521	-	1323	WP_045281263.1	pyrimidine utilization transport protein G	-
A4308_RS12835	2707543..2708037	-	495	WP_045281262.1	pyrimidine utilization flavin reductase protein F	-
A4308_RS12840	2708047..2708637	-	591	WP_045281261.1	malonic semialdehyde reductase	-
A4308_RS12845	2708647..2709447	-	801	WP_023338236.1	pyrimidine utilization protein D	-
A4308_RS12850	2709455..2709841	-	387	WP_045281260.1	pyrimidine utilization protein C	-
A4308_RS12855	2709853..2710542	-	690	WP_014883310.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19689.59 Da
• Isoelectric Point: 8.9464

>T62639 WP_176450254.1 NZ_CP015227:2705601-2706125 [Enterobacter sp. ODB01]
IVANLTIEMFSEGSDYDFRGFDCGEPSLNAFLTEHLVRQHKGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSKTQQ
RRIPYVNVPSVTLGRLAVHKDLQGKEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAKRFYLKQGFIPLTGENSHSL
FFPTKSIEQLFEQA

Nucleotide

• Length: 525 bp

>T62639 NZ_CP015227:2705601-2706125 [Enterobacter sp. ODB01]
ATCGTGGCAAATCTCACGATCGAAATGTTTTCAGAGGGAAGTGATTACGATTTCAGGGGCTTCGATTGTGGTGAACCCTC
GCTCAATGCCTTCCTGACGGAACATCTGGTGCGTCAGCACAAGGGACGGATATTGCGAGGTTACCTGCTTAAAGATCGTG
ACCATCCGCGTGTGCTGGGCTATTACACGTTGTCCGGAAGCTGCTTTGAAAAAGCGATGCTTCCTTCTAAGACCCAGCAG
CGTCGGATCCCCTATGTCAACGTACCCAGTGTGACGCTGGGGCGTCTGGCTGTGCATAAAGATCTACAGGGAAAGGAGTG
GGGAACAACGCTCGTCGCACATGCTATGCGCGTTGTGTATCTGGCATCGCAGGCGGTAGGGGTACACGGAATCTTTGTTG
ATGCTCTCAATGAACAGGCAAAACGATTCTATCTGAAGCAGGGTTTTATTCCGCTGACAGGTGAAAACAGCCATTCTCTT
TTCTTCCCCACAAAATCTATCGAACAACTCTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10278.72 Da
• Isoelectric Point: 5.8836

>AT62639 WP_014883303.1 NZ_CP015227:2705328-2705597 [Enterobacter sp. ODB01]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNESSWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT62639 NZ_CP015227:2705328-2705597 [Enterobacter sp. ODB01]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAGACAATCACCCAGTTTATGGTGAACAGTGCGTCAGAACGCGCTGCTGAAGTCATCGAACAACATCGGCGTC
TCGTATTAAACGAGTCATCATGGAATGCGGTGATGGACGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W0G3H3