Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 551271..551907 Replicon chromosome
Accession NZ_CP015222
Organism Bacillus subtilis strain HRBS-10TDI13

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag A4A60_RS02680 Protein ID WP_003156187.1
Coordinates 551557..551907 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag A4A60_RS02675 Protein ID WP_003225183.1
Coordinates 551271..551552 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4A60_RS02655 547630..548229 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
A4A60_RS02660 548324..548689 + 366 WP_014478897.1 holo-ACP synthase -
A4A60_RS02665 548855..549871 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
A4A60_RS02670 549986..551155 + 1170 WP_014478898.1 alanine racemase -
A4A60_RS02675 551271..551552 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
A4A60_RS02680 551557..551907 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
A4A60_RS02685 552023..552847 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
A4A60_RS02690 552852..553217 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
A4A60_RS02695 553221..553622 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
A4A60_RS02700 553634..554641 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
A4A60_RS02705 554710..555039 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
A4A60_RS02710 555036..555518 + 483 WP_003234299.1 anti-sigma B factor RsbW -
A4A60_RS02715 555484..556272 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
A4A60_RS02720 556272..556871 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T62630 WP_003156187.1 NZ_CP015222:551557-551907 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T62630 NZ_CP015222:551557-551907 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT62630 WP_003225183.1 NZ_CP015222:551271-551552 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT62630 NZ_CP015222:551271-551552 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References