Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 235331..235959 Replicon chromosome
Accession NZ_CP015218
Organism Leptospira tipperaryensis strain GWTS#1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7V3I0
Locus tag A0128_RS20470 Protein ID WP_069609615.1
Coordinates 235561..235959 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7V3I6
Locus tag A0128_RS20465 Protein ID WP_069609614.1
Coordinates 235331..235561 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0128_RS20430 230763..231389 + 627 Protein_210 YiiX family permuted papain-like enzyme -
A0128_RS20435 231478..232110 + 633 WP_069609608.1 DUF4236 domain-containing protein -
A0128_RS20440 232055..232618 + 564 WP_156781951.1 hypothetical protein -
A0128_RS20455 233923..234306 + 384 WP_069609612.1 type II toxin-antitoxin system RelE/ParE family toxin -
A0128_RS20460 234303..234584 + 282 WP_069609613.1 helix-turn-helix transcriptional regulator -
A0128_RS20465 235331..235561 + 231 WP_069609614.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A0128_RS20470 235561..235959 + 399 WP_069609615.1 type II toxin-antitoxin system VapC family toxin Toxin
A0128_RS20475 236294..236542 + 249 WP_069609616.1 ChpI protein -
A0128_RS20480 236529..236870 + 342 WP_069609617.1 type II toxin-antitoxin system PemK/MazF family toxin -
A0128_RS20485 237135..237347 + 213 WP_069609618.1 hypothetical protein -
A0128_RS20490 237360..238196 + 837 WP_069609619.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
A0128_RS22285 238668..239473 - 806 Protein_221 IS5 family transposase -
A0128_RS20500 239824..240549 + 726 WP_069609620.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 201400..239151 37751


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15149.71 Da        Isoelectric Point: 9.4041

>T62628 WP_069609615.1 NZ_CP015218:235561-235959 [Leptospira tipperaryensis]
MYLLDTNICIFLIKKKNAVLLGNLKKKINKDLFISSLTVAELEFGIQKSEHREKNKVALIEFLTIFNILSFSDKDAESYG
FIRADLEKRGSVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKDLKLEDWTQ

Download         Length: 399 bp

>T62628 NZ_CP015218:235561-235959 [Leptospira tipperaryensis]
ATGTATCTTCTGGATACCAATATTTGTATTTTTCTAATTAAAAAGAAAAACGCAGTTCTTTTAGGAAACCTTAAGAAAAA
AATAAATAAAGATTTATTTATTTCTTCTTTAACCGTCGCGGAGCTTGAATTTGGAATTCAGAAAAGTGAACATAGGGAAA
AAAACAAAGTTGCGCTGATTGAATTCTTAACAATTTTTAATATACTTTCTTTTTCTGATAAAGATGCCGAGTCTTATGGG
TTCATTAGGGCTGACTTAGAAAAAAGGGGAAGCGTAATTGGTTCAATAGATATGCTTCTTGCAGCTCAAGCAATTGCTAA
TAATTATATTTTTGTAACTAATAATACAAAAGAATTTAAACGAATTAAGGACTTAAAACTCGAAGATTGGACTCAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8699.97 Da        Isoelectric Point: 8.6588

>AT62628 WP_069609614.1 NZ_CP015218:235331-235561 [Leptospira tipperaryensis]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFLEEGREKPKSDKREKF

Download         Length: 231 bp

>AT62628 NZ_CP015218:235331-235561 [Leptospira tipperaryensis]
ATGCAAACAGCAAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTGCCAAAAGAATTTCAATTTACCGGAGATGA
CGTCCTTATTCAAAAAGTAGGAGAAGCGGTAATATTAGTTCCTAAGAATAAGGCATGGAATGTATTTTTGGAAGGATTAA
ATGGATTTTCAGATGATTTTCTGGAGGAGGGAAGAGAAAAACCGAAATCCGATAAAAGAGAAAAGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7V3I0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7V3I6

References