Detailed information of TA system TA062625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-doc
• Location: 113462..114074
• Replicon: plasmid a
• Accession: NZ_CP015212
• Organism: Cereibacter sphaeroides strain MBTLJ-13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q3IUZ4
• Locus tag: A3858_RS19990
• Protein ID: WP_011331447.1
• Coordinates: 113679..114074 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3IUZ5
• Locus tag: A3858_RS22855
• Protein ID: WP_011331446.1
• Coordinates: 113462..113689 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3858_RS22850	109683..110894	-	1212	WP_011331443.1	replication initiation protein RepC	-
A3858_RS19975	111077..112063	-	987	WP_011331444.1	plasmid partitioning protein RepB	-
A3858_RS19980	112063..113247	-	1185	WP_011331445.1	plasmid partitioning protein RepA	-
A3858_RS22855	113462..113689	+	228	WP_011331446.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A3858_RS19990	113679..114074	+	396	WP_011331447.1	PIN domain-containing protein	Toxin
A3858_RS19995	114154..115038	-	885	WP_061350894.1	recombinase family protein	-
A3858_RS20000	115751..116344	+	594	WP_011331449.1	biliverdin-producing heme oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..152978	152978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• SpoVT-AbrB domain (5-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14296.31 Da
• Isoelectric Point: 4.3422

>T62625 WP_011331447.1 NZ_CP015212:113679-114074 [Cereibacter sphaeroides]
MSAEFADTNVILYLLDNGPKADCAEEILGHRPRISVQVLNETLVNCRRKAGLSWEEAGAFLEGVRALCPVESLTIQTHDV
GRALAERYGFSIYDSIIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T62625 NZ_CP015212:113679-114074 [Cereibacter sphaeroides]
ATGAGCGCTGAGTTCGCGGACACGAACGTCATCCTTTACCTGCTCGATAACGGACCGAAGGCTGACTGTGCCGAGGAAAT
TCTGGGTCATAGGCCCCGGATCAGCGTTCAGGTCCTGAACGAGACGCTGGTGAATTGCCGCCGCAAAGCCGGCCTCAGTT
GGGAGGAAGCAGGAGCGTTTCTCGAAGGAGTGCGCGCCCTGTGTCCCGTCGAAAGCCTGACCATTCAGACCCATGACGTC
GGCCGCGCCTTGGCGGAACGCTACGGCTTCTCGATCTACGACTCGATTATCGTCGCCAGCGCTTTGGTCGCGGGGTGCAC
CACCCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTTGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8569.87 Da
• Isoelectric Point: 10.9144

>AT62625 WP_011331446.1 NZ_CP015212:113462-113689 [Cereibacter sphaeroides]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDHQVRVRRLARADEVLMGLRRFRGALPAAERLSRNDAHER

Nucleotide

• Length: 228 bp

>AT62625 NZ_CP015212:113462-113689 [Cereibacter sphaeroides]
ATGCAGGTCGCAAAATGGGGCAACTCGCTGGCCGTCCGCTTGCCCGCGGAGCTTGTTCGGGAACTGGGTCTCAAGGAAGG
CGATCAGATCGACTTGGTCAAGGACGACCACCAGGTCCGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTGCTGATGGGTC
TGCGCCGTTTCCGGGGCGCGTTGCCGGCTGCAGAGCGCCTTAGCCGCAACGACGCGCATGAGCGCTGA

Structures

Toxin

• AlphaFold DB: Q3IUZ4

Antitoxin

• AlphaFold DB: Q3IUZ5