Detailed information of TA system TA062624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1410031..1410581
• Replicon: chromosome
• Accession: NZ_CP015210
• Organism: Cereibacter sphaeroides strain MBTLJ-13

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q3J2R5
• Locus tag: A3858_RS06805
• Protein ID: WP_002719915.1
• Coordinates: 1410031..1410279 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q3J2R4
• Locus tag: A3858_RS06810
• Protein ID: WP_011337726.1
• Coordinates: 1410276..1410581 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3858_RS23210	1405445..1405738	+	294	WP_147175732.1	hypothetical protein	-
A3858_RS06775	1405735..1406523	+	789	WP_017140199.1	hypothetical protein	-
A3858_RS06780	1406538..1407191	+	654	WP_011337721.1	head decoration protein	-
A3858_RS06785	1407236..1407709	+	474	WP_017140200.1	hypothetical protein	-
A3858_RS06790	1407702..1408103	+	402	WP_011337723.1	hypothetical protein	-
A3858_RS06795	1408096..1408548	+	453	WP_002719913.1	hypothetical protein	-
A3858_RS06800	1408545..1408778	+	234	WP_011337724.1	tail protein X	-
A3858_RS06805	1410031..1410279	+	249	WP_002719915.1	BrnT family toxin	Toxin
A3858_RS06810	1410276..1410581	+	306	WP_011337726.1	BrnA antitoxin family protein	Antitoxin
A3858_RS06815	1411110..1411382	+	273	WP_011337727.1	hypothetical protein	-
A3858_RS22070	1411478..1412290	-	813	WP_023003613.1	hypothetical protein	-
A3858_RS23465	1412406..1412546	-	141	WP_002719918.1	hypothetical protein	-
A3858_RS06820	1412674..1412967	+	294	WP_011337728.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1400983..1412290	11307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (23-97)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8950.40 Da
• Isoelectric Point: 6.4624

>T62624 WP_002719915.1 NZ_CP015210:1410031-1410279 [Cereibacter sphaeroides]
MLVIVWDEPKRRSNLAKHGLDFADLDEEFFLSSVVVPASAGRHMAIGRLEDGTIAVVFAVLGAEGVSIISMRPASRKERS
LL

Nucleotide

• Length: 249 bp

>T62624 NZ_CP015210:1410031-1410279 [Cereibacter sphaeroides]
ATGTTGGTGATTGTATGGGATGAGCCAAAGCGGCGCAGCAACCTGGCGAAGCATGGGCTGGACTTCGCGGACCTAGACGA
GGAGTTCTTCCTGTCGTCGGTTGTCGTGCCAGCAAGCGCAGGCCGTCACATGGCGATCGGGCGCCTTGAGGATGGAACCA
TTGCCGTGGTCTTCGCGGTTCTGGGGGCCGAGGGCGTTTCGATCATCTCTATGCGCCCTGCGAGCCGAAAGGAGAGGAGC
CTTCTATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11061.61 Da
• Isoelectric Point: 10.5767

>AT62624 WP_011337726.1 NZ_CP015210:1410276-1410581 [Cereibacter sphaeroides]
MTKKRQLDTEETRIQRMIASDPDAPEATDAQLAQARPFTEAFPVLAEAMRRSAGGRPKLDAPKMSVSLRLDQDVVAHFKA
GGPGWQSRMNRALREAVGLSG

Nucleotide

• Length: 306 bp

>AT62624 NZ_CP015210:1410276-1410581 [Cereibacter sphaeroides]
ATGACGAAGAAGCGTCAACTCGACACGGAAGAGACCCGCATCCAGCGGATGATCGCGAGCGACCCGGATGCTCCCGAAGC
GACCGACGCGCAGCTTGCGCAGGCCCGACCCTTCACTGAGGCGTTTCCTGTGCTGGCCGAGGCCATGCGGCGCAGCGCAG
GCGGTCGCCCGAAGCTGGACGCGCCGAAAATGTCGGTTTCACTTCGCCTCGATCAGGACGTGGTGGCGCACTTCAAGGCA
GGCGGCCCCGGTTGGCAATCTCGGATGAACCGCGCCCTGCGGGAGGCGGTCGGCCTCTCCGGCTAG

Structures

Toxin

• AlphaFold DB: Q3J2R5

Antitoxin

• AlphaFold DB: Q3J2R4