Detailed information of TA system TA062623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1255877..1256652
• Replicon: chromosome
• Accession: NZ_CP015208
• Organism: Candidatus Rhodoluna planktonica strain MWH-Dar1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: A4Z71_RS06260
• Protein ID: WP_084028450.1
• Coordinates: 1256143..1256652 (+)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: A4Z71_RS06255
• Protein ID: WP_070955286.1
• Coordinates: 1255877..1256152 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4Z71_RS06245	1251432..1253975	-	2544	WP_070955041.1	hypothetical protein	-
A4Z71_RS06250	1254195..1255613	-	1419	WP_070955042.1	sigma-70 family RNA polymerase sigma factor	-
A4Z71_RS06255	1255877..1256152	+	276	WP_070955286.1	DUF1778 domain-containing protein	Antitoxin
A4Z71_RS06260	1256143..1256652	+	510	WP_084028450.1	GNAT family N-acetyltransferase	Toxin
A4Z71_RS06990	1256910..1257056	-	147	WP_202816202.1	DUF2256 domain-containing protein	-
A4Z71_RS06265	1257144..1258454	+	1311	WP_070955043.1	hypothetical protein	-
A4Z71_RS06270	1258577..1258897	+	321	WP_070955044.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (36-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18345.13 Da
• Isoelectric Point: 7.8028

>T62623 WP_084028450.1 NZ_CP015208:1256143-1256652 [Candidatus Rhodoluna planktonica]
MGVSLKQPRLLNATDQVDGFDSGSELIDAWFHKYSLIAQSAGTAKTFVVCTSEGEIAGFYSIATGQINHAEATERVCKGV
GQHSIPVIVLARLAVDLRFQGFGVGAGLLKDCAIRVGNIREQVGVRALVTHAKDEDAAKFYSRFGFVESPIAERQMMILM
KDLRGLIRS

Nucleotide

• Length: 510 bp

>T62623 NZ_CP015208:1256143-1256652 [Candidatus Rhodoluna planktonica]
TTGGGAGTAAGTCTCAAACAACCTCGGCTGCTGAACGCAACCGATCAGGTTGATGGTTTTGACTCAGGCTCAGAACTAAT
TGACGCATGGTTTCACAAATACTCGTTAATTGCGCAATCGGCAGGCACTGCAAAAACTTTCGTAGTTTGCACAAGCGAGG
GCGAAATTGCCGGTTTTTACAGCATCGCTACCGGGCAAATTAACCACGCCGAAGCAACCGAACGGGTTTGCAAAGGGGTT
GGGCAGCACTCCATCCCGGTAATTGTCTTAGCCCGACTGGCAGTTGATTTGCGTTTTCAGGGCTTCGGGGTTGGCGCAGG
GTTACTGAAAGATTGTGCGATTCGTGTGGGGAACATTCGCGAACAGGTTGGGGTGCGAGCACTAGTAACACACGCCAAAG
ACGAAGACGCGGCCAAGTTCTATAGTCGGTTCGGCTTTGTTGAGTCACCAATTGCTGAACGCCAAATGATGATTCTGATG
AAAGACCTTCGTGGGCTGATTCGGAGTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10168.55 Da
• Isoelectric Point: 5.1255

>AT62623 WP_070955286.1 NZ_CP015208:1255877-1256152 [Candidatus Rhodoluna planktonica]
MAESQTKGERIYTRVTPLEKRMIASAAKAQHQDVSAFMLESALREATNTLLDQRIFWADADAFAALEEDLARPAHTPAGL
IELLSRKAPWE

Nucleotide

• Length: 276 bp

>AT62623 NZ_CP015208:1255877-1256152 [Candidatus Rhodoluna planktonica]
ATGGCAGAATCACAAACAAAAGGTGAGCGCATCTACACCAGGGTGACGCCACTAGAAAAACGCATGATTGCTTCCGCCGC
CAAGGCGCAGCATCAAGACGTGTCGGCTTTCATGCTTGAAAGTGCTCTGCGCGAAGCTACCAACACCTTGCTTGATCAAC
GAATTTTTTGGGCCGATGCTGATGCCTTCGCGGCGCTCGAAGAAGATTTGGCACGGCCGGCACATACACCTGCTGGCTTG
ATTGAGTTGCTAAGTCGAAAAGCCCCTTGGGAGTAA