Detailed information of TA system TA062621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 437753..438285
• Replicon: plasmid pR8L1
• Accession: NZ_CP015203
• Organism: Rhodococcus sp. 008

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AOT96_RS32440
• Protein ID: WP_058228211.1
• Coordinates: 437986..438285 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AOT96_RS32435
• Protein ID: WP_058228212.1
• Coordinates: 437753..437986 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOT96_RS32415	434120..434902	+	783	WP_058228217.1	AAA family ATPase	-
AOT96_RS32425	435864..436832	+	969	WP_058228214.1	DUF2637 domain-containing protein	-
AOT96_RS32430	436867..437565	+	699	WP_058228213.1	TetR/AcrR family transcriptional regulator	-
AOT96_RS32435	437753..437986	+	234	WP_058228212.1	hypothetical protein	Antitoxin
AOT96_RS32440	437986..438285	+	300	WP_058228211.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AOT96_RS32445	438314..440164	+	1851	WP_058228210.1	hypothetical protein	-
AOT96_RS32450	440167..440964	+	798	WP_058228209.1	hypothetical protein	-
AOT96_RS32455	441237..442307	-	1071	WP_058228208.1	DUF1016 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..694228	694228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10624.28 Da
• Isoelectric Point: 5.5813

>T62621 WP_058228211.1 NZ_CP015203:437986-438285 [Rhodococcus sp. 008]
MRPIHEADLDKVRPVLVLTREIIRPHLKHVTVAPITTVMRGLSTEVPVGIANGVDADSVISCENVTTIPSSALGRNIGYL
LPAQEESLSAAVRAAFDLE

Nucleotide

• Length: 300 bp

>T62621 NZ_CP015203:437986-438285 [Rhodococcus sp. 008]
ATGCGACCGATACACGAAGCTGATCTCGACAAAGTCCGCCCCGTGCTGGTGCTGACCCGAGAAATCATCCGCCCGCACCT
CAAACACGTCACAGTTGCCCCCATCACCACCGTGATGCGAGGACTGTCCACCGAGGTGCCAGTCGGAATAGCAAACGGAG
TAGACGCCGACTCAGTGATCAGCTGCGAAAACGTCACCACCATCCCCAGCAGTGCACTCGGGCGCAACATCGGATACCTA
CTTCCTGCGCAAGAAGAATCGCTGTCCGCCGCCGTCCGCGCTGCGTTCGATCTCGAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8572.71 Da
• Isoelectric Point: 7.3215

>AT62621 WP_058228212.1 NZ_CP015203:437753-437986 [Rhodococcus sp. 008]
MSTQIAVRLPDEIVAFLDSEVREQRATSRASLVLRALERERRRQAAARDAEILAHATAHDELIPLVNHTTKQSTGLA

Nucleotide

• Length: 234 bp

>AT62621 NZ_CP015203:437753-437986 [Rhodococcus sp. 008]
ATGAGCACGCAAATCGCGGTCCGCCTACCGGACGAGATCGTGGCATTTCTCGACTCCGAGGTCAGAGAGCAACGCGCCAC
ATCACGCGCCTCGCTGGTGCTACGGGCGCTCGAACGCGAGCGCCGCCGACAGGCCGCCGCACGCGACGCCGAAATCCTCG
CTCACGCAACCGCCCATGACGAACTCATTCCACTGGTCAATCACACAACGAAACAATCGACGGGTCTCGCCTGA