Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 414816..415381 Replicon plasmid pR8L1
Accession NZ_CP015203
Organism Rhodococcus sp. 008

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag AOT96_RS32315 Protein ID WP_058228574.1
Coordinates 414816..415172 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag AOT96_RS32320 Protein ID WP_058228575.1
Coordinates 415169..415381 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOT96_RS35055 410323..411270 + 948 Protein_396 RNA polymerase sigma factor -
AOT96_RS32290 411592..411852 + 261 WP_058228569.1 ribbon-helix-helix domain-containing protein -
AOT96_RS32295 411849..412085 + 237 WP_058228570.1 hypothetical protein -
AOT96_RS35570 412703..413164 + 462 WP_197523656.1 hypothetical protein -
AOT96_RS32310 414233..414622 + 390 WP_058228573.1 helix-turn-helix domain-containing protein -
AOT96_RS32315 414816..415172 - 357 WP_058228574.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AOT96_RS32320 415169..415381 - 213 WP_058228575.1 ribbon-helix-helix domain-containing protein Antitoxin
AOT96_RS35900 415900..416084 - 185 Protein_403 hypothetical protein -
AOT96_RS32330 416599..417632 + 1034 Protein_404 diiron oxygenase -
AOT96_RS32345 418799..418987 - 189 WP_058228228.1 hypothetical protein -
AOT96_RS32350 419230..419421 - 192 WP_058228227.1 hypothetical protein -
AOT96_RS32355 419587..420366 - 780 WP_058228226.1 SigB/SigF/SigG family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..694228 694228


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-39)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12909.63 Da        Isoelectric Point: 6.7604

>T62620 WP_058228574.1 NZ_CP015203:c415172-414816 [Rhodococcus sp. 008]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANVTAENHGRGVITVVPLTTNTAHVFPFQVMVESDESGLARASKAQA
EQVRSVSIRHLDPEPVGKVGMETFAALEDALRLHLDLQ

Download         Length: 357 bp

>T62620 NZ_CP015203:c415172-414816 [Rhodococcus sp. 008]
ATGATTCGCCGCGCGCACATTCACTGGGTGGATCTCGATCCCGCGCAGGGCAGTGAAGCGGCGAAACGACGACCGGCGGT
GGTGGTGAGCAACGACCGGGCCAATGTTACTGCAGAAAATCACGGCAGGGGCGTGATCACCGTGGTGCCGTTGACTACCA
ATACTGCGCACGTGTTTCCGTTTCAGGTGATGGTCGAGTCCGACGAATCAGGTCTGGCGCGAGCGTCGAAAGCGCAAGCG
GAGCAAGTGCGGTCGGTATCGATTCGGCATCTGGATCCTGAACCTGTCGGCAAGGTCGGGATGGAGACTTTCGCCGCGCT
CGAGGATGCACTGCGGTTGCACCTCGATCTGCAGTAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7652.40 Da        Isoelectric Point: 4.1824

>AT62620 WP_058228575.1 NZ_CP015203:c415381-415169 [Rhodococcus sp. 008]
MTTKFTISLPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVTADAE

Download         Length: 213 bp

>AT62620 NZ_CP015203:c415381-415169 [Rhodococcus sp. 008]
ATGACCACGAAATTCACCATAAGTCTCCCTGACGAGGATGCACACCTACTCGATGAGTATGTGCGTGATCACGGACTTCG
TGGCCGGTCGGCAGGAATTCGAGCAGCACTCCGCCTACTCTCGACGTCCGGACTCGAACGTGACTACGCCGCAGCCTTTG
CTGAAGGTGACGTAGATGAAGTGTGGGACGCTGTCACTGCAGACGCCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References