Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 6253796..6254382 Replicon chromosome
Accession NZ_CP015202
Organism Pseudomonas putida B6-2

Toxin (Protein)


Gene name PumA Uniprot ID A0A2I0CCV1
Locus tag KKK_RS28285 Protein ID WP_004576348.1
Coordinates 6254089..6254382 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID I3UY28
Locus tag KKK_RS28280 Protein ID WP_004576347.1
Coordinates 6253796..6254092 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKK_RS28255 6249190..6249543 + 354 WP_019750494.1 hypothetical protein -
KKK_RS28260 6249566..6250747 + 1182 WP_110096821.1 phage major capsid protein -
KKK_RS28265 6251872..6252105 + 234 WP_019750492.1 hypothetical protein -
KKK_RS28270 6252488..6253135 + 648 WP_029380519.1 serine protease -
KKK_RS28275 6253306..6253785 + 480 WP_019750490.1 GNAT family N-acetyltransferase -
KKK_RS28280 6253796..6254092 - 297 WP_004576347.1 putative addiction module antidote protein Antitoxin
KKK_RS28285 6254089..6254382 - 294 WP_004576348.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KKK_RS28290 6254606..6255499 + 894 WP_019750488.1 effector locus protein -
KKK_RS28295 6255536..6256303 - 768 WP_019750487.1 phosphonate ABC transporter, permease protein PhnE -
KKK_RS28300 6256300..6257127 - 828 WP_004576350.1 ABC transporter permease subunit -
KKK_RS28305 6257121..6257930 - 810 WP_019750486.1 phosphonate ABC transporter ATP-binding protein -
KKK_RS28310 6257927..6258781 - 855 WP_014861459.1 putative selenate ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 6242926..6300954 58028


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(2-96)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10751.55 Da        Isoelectric Point: 10.8302

>T62618 WP_004576348.1 NZ_CP015202:c6254382-6254089 [Pseudomonas putida B6-2]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
TQAADILNARKLLKEMK

Download         Length: 294 bp

>T62618 NZ_CP015202:c6254382-6254089 [Pseudomonas putida B6-2]
ATGTTTCTCGTTGAACAGACGGAAAGCTTTGCCGCTTGGCTATCGAGCTTGAGAGATTTGAGGGCCAGGCTGGCGATAGG
TCGTAGGCTGGAGCGAGCAGCGGCGGGCAATCTTGGTGACTTCAAGTGGCTTGGAGGCGGTATTGGTGAGCTTCGCATCG
ACGTAGCTGCCGGTTATCGCGTCTATTTCACGCAAAAGGGGCAGAGGTTGGTTTTACTACTGGTCGGCGGCGACAAGTCC
ACCCAGGCCGCCGACATCCTCAACGCCAGAAAGCTGCTGAAGGAGATGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10676.33 Da        Isoelectric Point: 6.7249

>AT62618 WP_004576347.1 NZ_CP015202:c6254092-6253796 [Pseudomonas putida B6-2]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Download         Length: 297 bp

>AT62618 NZ_CP015202:c6254092-6253796 [Pseudomonas putida B6-2]
ATGAGCGAAAAATTGATCCCGTTTGATATGGCAGCCTTGTTGAGCAGCGATGAGGCAATCAGTGAATACCTTAGCCAGGT
GCTATCAAATGGCGATACCGAGGAAATCATTCGAGCACTTGGCCATATCGCTCGTGCACGGGGCATGACACATATTGCTA
CGGAGAGTGGGCTGGGTAGAGAAAGCCTGTACAAGGCGCTGAGCCCTGGCGCGAAGCCTCGTTTTGACACTGTATTGAGG
GTAATTCGTGCTCTGGGGGTGGACCTTCTCGTTCAGCCACATGCAATGACCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I0CCV1

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0CD26

References