Detailed information of TA system TA062617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 5467594..5469323
• Replicon: chromosome
• Accession: NZ_CP015202
• Organism: Pseudomonas putida B6-2

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: KKK_RS24735
• Protein ID: WP_019752695.1
• Coordinates: 5468682..5469323 (-)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: I3UUH2
• Locus tag: KKK_RS24730
• Protein ID: WP_014754747.1
• Coordinates: 5467594..5468682 (-)
• Length: 363 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKK_RS24715	5463224..5464042	-	819	WP_173881454.1	HDOD domain-containing protein	-
KKK_RS24720	5464218..5465414	+	1197	WP_004575470.1	class I SAM-dependent rRNA methyltransferase	-
KKK_RS24725	5465685..5467526	+	1842	WP_003249056.1	dihydroxy-acid dehydratase	-
KKK_RS24730	5467594..5468682	-	1089	WP_014754747.1	macro domain-containing protein	Antitoxin
KKK_RS24735	5468682..5469323	-	642	WP_019752695.1	DUF4433 domain-containing protein	Toxin
KKK_RS24740	5469320..5469898	-	579	WP_019752696.1	DUF4433 domain-containing protein	-
KKK_RS24745	5469996..5471051	-	1056	WP_019752697.1	phosphorylcholine phosphatase	-
KKK_RS24750	5471296..5472687	+	1392	WP_003249050.1	L-cystine transporter	-
KKK_RS24755	5472819..5473334	-	516	WP_019752698.1	dihydrofolate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pilQ / algC	5413196..5881989	468793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24591.17 Da
• Isoelectric Point: 9.3541

>T62617 WP_019752695.1 NZ_CP015202:c5469323-5468682 [Pseudomonas putida B6-2]
MIYSSLNPSKALIWRIVHRDNLSWVLDHGLHAVSSGKLSSTYVGIGNPELISKRAQRRVPLEPNGTLADYVPFYFTPFSV
MMKNIHSGWGVPQRRNEEIVILVSSLYHAQSLGLQFVFTDAHAYPHWAGYYSDLTQLDKIDWEILQRRDFKRDPDDPRKM
ERYQAEALIHGHVPVAALLGIVCYNEALKGRVDKEVQQRRLNLPVYARPGWYF

Nucleotide

• Length: 642 bp

>T62617 NZ_CP015202:c5469323-5468682 [Pseudomonas putida B6-2]
ATGATTTATTCCAGTCTCAACCCATCCAAGGCACTCATCTGGCGAATTGTTCACCGCGATAACCTGTCGTGGGTTCTGGA
TCATGGGCTGCACGCTGTCTCCTCCGGGAAGCTTTCCAGCACCTACGTTGGAATTGGCAATCCAGAGCTGATCAGCAAGC
GAGCGCAACGCCGTGTGCCTCTGGAACCCAATGGGACGCTGGCGGATTATGTACCGTTCTACTTCACCCCGTTCTCCGTG
ATGATGAAGAATATTCATTCGGGGTGGGGTGTTCCTCAGCGGCGGAACGAAGAGATCGTGATTCTTGTCTCCAGCCTGTA
CCATGCGCAGTCGCTGGGTTTGCAGTTTGTGTTCACGGATGCCCATGCCTACCCGCATTGGGCCGGCTACTACAGTGACC
TCACGCAGCTTGACAAAATCGATTGGGAGATTTTGCAGCGCAGGGATTTCAAACGTGACCCTGATGACCCTCGGAAAATG
GAGCGTTATCAGGCGGAGGCCCTTATCCATGGACATGTCCCAGTTGCTGCCCTTCTGGGAATTGTCTGCTACAACGAAGC
TTTGAAGGGGCGTGTCGACAAGGAGGTTCAGCAACGTCGATTGAATCTCCCTGTGTATGCTCGTCCTGGGTGGTACTTCT
AA

Antitoxin

Protein

• Length: 363 a.a.
• Molecular weight: 40625.75 Da
• Isoelectric Point: 6.5916

>AT62617 WP_014754747.1 NZ_CP015202:c5468682-5467594 [Pseudomonas putida B6-2]
MIHFMQGNLLEAKAEALVNTVNTVGVMGKGIALMFKERFVENYRQYAAACKAKEVVTGKMFVTEVRELDGPRWIVNFPTK
RHWRSPSQIGWIVEGLHDLRNFIINNQVASIAIPPLGAGNGGLSWAAVREQIQRVLGDLDADVFVFEPTANYQNVSKRSG
VEVLTPARALIAELVRRYWVLGMECSLLEVQKLAWFLERSIERFTPGINPLELKFVAHKYGPYANRLEHLLDNLDGSYLH
CRKRISDAGPLDVIWFDEGRKSFLQAYLNSEAKAYLPALEFTAELIDGFESPFGMELLATVDWLLHEDGVEPTVPAVREH
LKNWSGGVGAAARKSKLFDDEALGIALQKLSMFRSDPATSLH

Nucleotide

• Length: 1089 bp

>AT62617 NZ_CP015202:c5468682-5467594 [Pseudomonas putida B6-2]
ATGATCCACTTTATGCAAGGTAACCTGCTGGAGGCCAAGGCCGAAGCGCTGGTTAACACAGTTAACACCGTAGGGGTGAT
GGGGAAAGGCATCGCGCTGATGTTCAAGGAGCGTTTTGTCGAGAACTATCGCCAGTATGCTGCTGCCTGCAAAGCGAAAG
AGGTAGTTACCGGGAAGATGTTTGTTACTGAAGTGCGTGAGCTGGACGGTCCACGCTGGATTGTGAATTTTCCAACCAAG
CGGCATTGGCGCTCACCTTCGCAAATCGGCTGGATCGTCGAGGGCCTTCATGATTTGCGCAACTTCATTATCAATAACCA
GGTGGCATCGATTGCTATCCCTCCACTTGGTGCTGGTAATGGAGGGTTGTCTTGGGCTGCGGTACGAGAACAGATTCAGA
GGGTTCTGGGTGATCTGGACGCGGATGTTTTCGTTTTCGAGCCTACGGCAAATTATCAAAACGTTTCCAAGCGCAGTGGT
GTTGAGGTTTTGACCCCGGCTCGAGCCTTGATTGCAGAGCTTGTGCGCCGCTATTGGGTGCTGGGGATGGAGTGCAGCTT
GCTCGAAGTCCAGAAGCTTGCCTGGTTTCTGGAGCGATCAATCGAGCGGTTCACGCCAGGCATCAACCCATTGGAATTGA
AGTTTGTCGCTCATAAGTATGGTCCTTACGCCAACCGGCTTGAGCACCTTCTAGATAACCTGGATGGCAGTTATTTACAC
TGCCGAAAGCGGATCAGCGATGCAGGCCCTCTGGACGTTATCTGGTTTGATGAGGGGCGCAAGTCATTTTTACAGGCGTA
TCTGAACAGTGAAGCCAAGGCCTATTTGCCGGCGTTGGAGTTCACTGCCGAGTTGATTGATGGTTTCGAGTCTCCGTTTG
GTATGGAGCTGCTGGCCACCGTTGATTGGTTATTGCATGAAGATGGTGTTGAGCCGACAGTCCCTGCAGTTCGCGAACAC
CTGAAAAATTGGTCAGGAGGGGTAGGTGCCGCTGCTCGAAAAAGCAAACTGTTCGATGACGAGGCACTTGGAATCGCTCT
TCAAAAGCTGTCAATGTTTCGATCCGACCCAGCCACCTCGCTTCACTGA

Structures

Antitoxin

• AlphaFold DB: I3UUH2