Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 4659818..4660391 Replicon chromosome
Accession NZ_CP015202
Organism Pseudomonas putida B6-2

Toxin (Protein)


Gene name relE Uniprot ID W5IUR0
Locus tag KKK_RS21145 Protein ID WP_009618209.1
Coordinates 4659818..4660105 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID W5ISI7
Locus tag KKK_RS21150 Protein ID WP_009618210.1
Coordinates 4660092..4660391 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKK_RS21120 4655460..4657475 - 2016 WP_019751272.1 DNA topoisomerase III -
KKK_RS21125 4657770..4658153 - 384 WP_016852001.1 single-stranded DNA-binding protein -
KKK_RS21130 4658150..4658698 - 549 WP_017244812.1 DUF3158 family protein -
KKK_RS21135 4658695..4659474 - 780 WP_017244811.1 TIGR03761 family integrating conjugative element protein -
KKK_RS21140 4659598..4659789 - 192 WP_011805449.1 hypothetical protein -
KKK_RS21145 4659818..4660105 - 288 WP_009618209.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KKK_RS21150 4660092..4660391 - 300 WP_009618210.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KKK_RS21155 4660519..4661712 - 1194 WP_017244810.1 hypothetical protein -
KKK_RS21160 4661715..4662275 - 561 WP_018076214.1 DUF2857 domain-containing protein -
KKK_RS21165 4662293..4663951 - 1659 WP_018076213.1 ParB family protein -
KKK_RS21170 4663944..4664180 - 237 WP_013982111.1 type II toxin-antitoxin system HicA family toxin -
KKK_RS21175 4664164..4665030 - 867 WP_018076212.1 ParA family protein -
KKK_RS21180 4665071..4665289 - 219 WP_011829925.1 AlpA family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4551246..4667612 116366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-90)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-58)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10854.46 Da        Isoelectric Point: 8.4628

>T62616 WP_009618209.1 NZ_CP015202:c4660105-4659818 [Pseudomonas putida B6-2]
VRVLEWREAARADLLAIVDYISDDNPDAAQRLKDDIEAKAAKLPERPKLYRPGRVAGTREMVVRANYVVVYMEDTRAVSI
LRVLHAAQQWPPARE

Download         Length: 288 bp

>T62616 NZ_CP015202:c4660105-4659818 [Pseudomonas putida B6-2]
GTGCGCGTACTTGAGTGGCGGGAAGCTGCCCGCGCCGACCTGCTGGCGATTGTTGACTACATCTCGGACGACAACCCCGA
TGCCGCGCAGCGGTTGAAGGACGACATCGAGGCCAAGGCCGCGAAGCTGCCGGAGCGCCCGAAGCTCTACCGGCCTGGCC
GCGTGGCCGGCACGCGGGAGATGGTCGTGCGTGCGAACTACGTGGTGGTCTACATGGAGGACACCCGCGCGGTGTCCATC
CTGCGCGTGTTGCACGCCGCGCAGCAGTGGCCGCCGGCACGGGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10816.46 Da        Isoelectric Point: 8.6579

>AT62616 WP_009618210.1 NZ_CP015202:c4660391-4660092 [Pseudomonas putida B6-2]
MAAQTSMLHIRVDDKLKADAAEKLAGVGLTVSDAVRILLTRVVKEGALPAGLTADPEAYDAWFRAKVQEALADTRPPVPH
QQVMDEAQALIDRKRRART

Download         Length: 300 bp

>AT62616 NZ_CP015202:c4660391-4660092 [Pseudomonas putida B6-2]
ATGGCCGCACAGACTTCGATGCTGCACATCCGCGTGGACGATAAGCTCAAGGCCGATGCAGCCGAGAAACTCGCCGGGGT
CGGCCTCACCGTTTCGGACGCCGTGCGCATCCTGCTGACCCGTGTGGTCAAGGAAGGCGCACTGCCGGCTGGCCTCACGG
CCGACCCGGAGGCCTACGATGCCTGGTTCCGGGCGAAGGTCCAGGAGGCGCTGGCCGACACACGGCCGCCGGTGCCGCAC
CAGCAGGTCATGGATGAGGCGCAGGCCCTGATCGACAGGAAGCGGCGTGCGCGTACTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R9QF16

Antitoxin

Source ID Structure
AlphaFold DB A0A5R9QE11

References