Detailed information of TA system TA062612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 998791..999830
• Replicon: chromosome
• Accession: NZ_CP015202
• Organism: Pseudomonas putida B6-2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: KKK_RS04400
• Protein ID: WP_019750859.1
• Coordinates: 998791..999366 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A173HI04
• Locus tag: KKK_RS04405
• Protein ID: WP_003251469.1
• Coordinates: 999363..999830 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKK_RS04390	994408..995643	+	1236	WP_029380557.1	FAD-dependent oxidoreductase	-
KKK_RS04395	995647..998577	-	2931	WP_019750858.1	aminotransferase	-
KKK_RS04400	998791..999366	-	576	WP_019750859.1	PIN domain-containing protein	Toxin
KKK_RS04405	999363..999830	-	468	WP_003251469.1	helix-turn-helix domain-containing protein	Antitoxin
KKK_RS04410	999975..1001582	-	1608	WP_019750860.1	ABC transporter ATP-binding protein	-
KKK_RS04415	1001584..1002603	-	1020	WP_003251467.1	ABC transporter permease	-
KKK_RS04420	1002605..1003678	-	1074	WP_003251466.1	microcin C ABC transporter permease YejB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21683.92 Da
• Isoelectric Point: 5.5710

>T62612 WP_019750859.1 NZ_CP015202:c999366-998791 [Pseudomonas putida B6-2]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFPKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHNVQQYLDRLLQQKLPETVKLLSAFKFLL

Nucleotide

• Length: 576 bp

>T62612 NZ_CP015202:c999366-998791 [Pseudomonas putida B6-2]
ATGAAGCACTCGCCCTTCACGGCCATATACGACGCGAATGTACTTTACCCTGCCCCACTGCGTGATTTCCTGATGAACCT
AGCGCTGACGGGCATCTACCGGGCACGTTGGTCAGCGAGCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGAC
CTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTAATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTAC
GATTCACTGGTCGAAGGGCTGGACTTGCCAGATCGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTC
GGTCATCGTCACCTTCAATTTGAAGGATTTTCCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACG
ACTTCATCGCTGACCTTTGGGACCTGGACAAGGCAGCAGTCCTCGAAGCTGCCCAGCGGCAGCGAATATCACTGAAGAAC
CCACCGCACAACGTACAGCAGTATCTCGACAGGCTGCTTCAGCAGAAGCTTCCCGAGACAGTGAAACTGCTTTCAGCATT
CAAGTTCCTCCTCTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17229.71 Da
• Isoelectric Point: 6.6505

>AT62612 WP_003251469.1 NZ_CP015202:c999830-999363 [Pseudomonas putida B6-2]
MTTANLSRHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRTAMDALAAQAQELKMGYE

Nucleotide

• Length: 468 bp

>AT62612 NZ_CP015202:c999830-999363 [Pseudomonas putida B6-2]
ATGACCACCGCCAACCTCTCTCGCCATGTGCTTCCTGATGAGAAAGAAATTGCCGTGGCAGTCGAGTCAAGCCGCACGCT
GGCAGCGTTCCTCTCGACAAAATCAGATACCCAACGGATCGAACTGCTCAACGAGGAAAATCAGCGCCAGGTTGTTGAAG
TACCTACTTTCGCCCTACGCCTATTCGGCGAGATTCTCAGCGAACTGGCCCTGGGTAACTCAGTCAAAGTCGTTCCAATC
CACGCCGAACTAACTACCCAGGAAGCAGCAGACCTGCTTAACGTCTCCCGGCCGCACTTGGTAAAGCTGCTCGACGAAGC
GGTGATTCCGCACACCAAGACAGGCCGCCATCGTCGTGTCAAATTCTCCGACCTGGTTGCCTACAAGGACAAACGTGATG
GGGTGAGCCGGACTGCGATGGATGCGCTAGCAGCCCAAGCCCAGGAATTGAAGATGGGGTACGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A173HI04