Detailed information of TA system TA062609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 89982..90648
• Replicon: chromosome
• Accession: NZ_CP015196
• Organism: Streptococcus marmotae strain HTS5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: A4H00_RS00400
• Protein ID: WP_067085951.1
• Coordinates: 90469..90648 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A4H00_RS00395
• Protein ID: WP_067085947.1
• Coordinates: 89982..90431 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4H00_RS00365	85063..86514	+	1452	WP_067085929.1	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase	-
A4H00_RS00370	87077..87796	-	720	WP_067085933.1	rRNA pseudouridine synthase	-
A4H00_RS00375	87923..88627	-	705	WP_067085936.1	glucosamine-6-phosphate deaminase	-
A4H00_RS00385	89258..89605	-	348	WP_067085942.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A4H00_RS00390	89595..89882	-	288	WP_067085945.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
A4H00_RS00395	89982..90431	-	450	WP_067085947.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A4H00_RS00400	90469..90648	-	180	WP_067085951.1	type II toxin-antitoxin system HicA family toxin	Toxin
A4H00_RS00405	90834..91247	-	414	WP_067085954.1	hypothetical protein	-
A4H00_RS00410	91228..91650	-	423	WP_067085956.1	hypothetical protein	-
A4H00_RS00415	91696..91974	-	279	WP_067085959.1	hypothetical protein	-
A4H00_RS00420	91964..92302	-	339	WP_067085961.1	replication protein	-
A4H00_RS00425	92335..92913	-	579	WP_157770960.1	hypothetical protein	-
A4H00_RS00430	93163..94419	-	1257	WP_067085967.1	ISL3 family transposase	-
A4H00_RS00435	94602..94853	-	252	WP_067085970.1	hypothetical protein	-
A4H00_RS00440	94850..95101	-	252	WP_067085973.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	89982..126919	36937
	-	flank	IS/Tn	-	-	93163..94419	1256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (18-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 10.9086

>T62609 WP_067085951.1 NZ_CP015196:c90648-90469 [Streptococcus marmotae]
MPMTQKEMVKLLIANGWTKTKGGKGSHIKMEKQGERPITVPHGELNKYTERGIRKQAGI

Nucleotide

• Length: 180 bp

>T62609 NZ_CP015196:c90648-90469 [Streptococcus marmotae]
ATGCCGATGACACAAAAAGAGATGGTAAAACTCCTAATCGCTAATGGTTGGACGAAGACCAAGGGCGGTAAAGGCTCCCA
CATCAAAATGGAAAAGCAAGGAGAAAGACCTATCACAGTCCCGCATGGTGAGCTGAACAAGTACACCGAACGCGGGATAA
GAAAGCAAGCGGGCATTTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16649.82 Da
• Isoelectric Point: 4.1405

>AT62609 WP_067085947.1 NZ_CP015196:c90431-89982 [Streptococcus marmotae]
MLVTYPALFYYDDTNGTNVPYFVHFPDIDGSGTQGTSLSDAMAMASEYLGIMVAYYIESNRELPKPSEINNLSLMENNPF
KDEPDFLSYDPGKSFVSLVMVDVAEYLGSQEPIKKTLTIPRWADKLGRELGLNFSQTLTEAIADKKIQA

Nucleotide

• Length: 450 bp

>AT62609 NZ_CP015196:c90431-89982 [Streptococcus marmotae]
ATGTTAGTTACTTATCCAGCCTTATTTTATTATGATGATACAAATGGGACTAACGTGCCTTACTTTGTACATTTTCCAGA
CATTGATGGTTCAGGTACTCAAGGCACAAGCCTTTCGGACGCTATGGCTATGGCGTCGGAATATCTTGGTATTATGGTCG
CTTACTACATTGAAAGCAATAGAGAGCTTCCCAAACCATCAGAAATTAACAACCTATCACTAATGGAAAATAACCCGTTT
AAAGATGAGCCCGATTTTTTATCTTATGACCCTGGAAAATCTTTCGTCTCCCTTGTTATGGTTGATGTTGCGGAGTATCT
AGGAAGCCAAGAGCCGATAAAAAAGACTTTAACAATCCCGCGTTGGGCAGACAAATTGGGGCGTGAGTTAGGTTTGAACT
TCTCTCAAACACTCACCGAAGCCATTGCAGATAAAAAAATACAAGCCTAG