Detailed information of TA system TA062600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 402451..403048
• Replicon: chromosome
• Accession: NZ_CP015193
• Organism: Thermococcus chitonophagus strain GC74

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A3L04_RS02195
• Protein ID: WP_084448844.1
• Coordinates: 402451..402840 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A160VU82
• Locus tag: A3L04_RS02200
• Protein ID: WP_068576321.1
• Coordinates: 402827..403048 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3L04_RS02175 (A3L04_02150)	398008..399288	+	1281	Protein_434	argininosuccinate lyase	-
A3L04_RS02180 (A3L04_02155)	399297..400121	+	825	WP_068576315.1	lysine biosynthesis protein LysX	-
A3L04_RS02185 (A3L04_02160)	400118..401722	-	1605	WP_068576317.1	radical SAM protein	-
A3L04_RS02190 (A3L04_02165)	401862..402482	+	621	WP_068576318.1	METTL5 family protein	-
A3L04_RS02195 (A3L04_02170)	402451..402840	-	390	WP_084448844.1	type II toxin-antitoxin system VapC family toxin	Toxin
A3L04_RS02200 (A3L04_02175)	402827..403048	-	222	WP_068576321.1	hypothetical protein	Antitoxin
A3L04_RS02205 (A3L04_02180)	403090..405417	-	2328	WP_068576323.1	DNA polymerase	-
A3L04_RS02210 (A3L04_02185)	405512..406324	+	813	WP_068576325.1	MBL fold metallo-hydrolase	-
A3L04_RS02215 (A3L04_02190)	406321..407061	-	741	WP_068576327.1	ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• Putative antitoxin VapB3-like (1-73)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14595.96 Da
• Isoelectric Point: 4.7712

>T62600 WP_084448844.1 NZ_CP015193:c402840-402451 [Thermococcus chitonophagus]
VIVIDASALVMVVLQEPGWEKVPIGSEVATLDYAYVEGMNAIWKAVRWRELTREQGRMKITVLRMMKSSITTYRTEDFFE
RGLEIALDEGIAVYDAFYIALAESLDAKLVTADEKQYKAAKNYVPAKLV

Nucleotide

• Length: 390 bp

>T62600 NZ_CP015193:c402840-402451 [Thermococcus chitonophagus]
GTGATAGTCATTGATGCATCCGCCCTGGTTATGGTAGTGCTTCAAGAGCCAGGATGGGAGAAAGTGCCAATTGGATCAGA
AGTAGCTACTTTGGACTACGCATACGTAGAAGGAATGAACGCCATATGGAAGGCCGTGCGATGGAGAGAACTTACAAGAG
AGCAGGGAAGGATGAAAATAACCGTGCTTAGGATGATGAAAAGCTCAATAACAACTTACAGAACAGAAGATTTCTTCGAA
AGAGGGTTGGAGATAGCGCTTGACGAAGGAATAGCAGTATACGATGCCTTTTACATAGCCCTAGCCGAGTCCTTGGATGC
GAAGTTAGTTACGGCCGATGAGAAGCAGTACAAAGCAGCTAAGAACTACGTCCCCGCGAAGCTCGTATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8710.98 Da
• Isoelectric Point: 9.7232

>AT62600 WP_068576321.1 NZ_CP015193:c403048-402827 [Thermococcus chitonophagus]
MAVLSIRIPDELKEKMKEFDINWSEEIRRFIKERIEYEERKRTLEKALELAKKSGSVSKGFSARAVREDRDSH

Nucleotide

• Length: 222 bp

>AT62600 NZ_CP015193:c403048-402827 [Thermococcus chitonophagus]
ATGGCGGTACTAAGCATAAGGATTCCGGATGAGCTAAAAGAGAAGATGAAGGAGTTCGACATAAACTGGAGCGAGGAGAT
CAGGAGGTTCATAAAAGAAAGGATAGAGTACGAGGAAAGAAAGAGAACCCTCGAGAAAGCCTTAGAGCTAGCTAAAAAAA
GTGGAAGTGTGAGTAAGGGTTTCTCAGCAAGAGCAGTGAGGGAGGATCGTGATAGTCATTGA

Structures

Antitoxin

• AlphaFold DB: A0A160VU82