62595

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2377784..2378762 Replicon chromosome
Accession NZ_CP015176
Organism Bacillus thuringiensis serovar alesti strain BGSC 4C1

Toxin (Protein)


Gene name spoIISA Uniprot ID B7IX38
Locus tag Bt4C1_RS12295 Protein ID WP_000624981.1
Coordinates 2377784..2378521 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID B7IX39
Locus tag Bt4C1_RS12300 Protein ID WP_000237817.1
Coordinates 2378634..2378762 (+) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Bt4C1_RS12270 2373379..2373786 + 408 WP_000072495.1 VOC family protein -
Bt4C1_RS12275 2374130..2374909 + 780 WP_063675368.1 class I SAM-dependent methyltransferase -
Bt4C1_RS12280 2375063..2375908 - 846 Protein_2405 alpha-keto acid decarboxylase family protein -
Bt4C1_RS30175 2375884..2376052 - 169 Protein_2406 hypothetical protein -
Bt4C1_RS12285 2376160..2376642 + 483 WP_000191905.1 MarR family winged helix-turn-helix transcriptional regulator -
Bt4C1_RS12290 2376808..2377545 + 738 WP_000594159.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
Bt4C1_RS12295 2377784..2378521 + 738 WP_000624981.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
Bt4C1_RS12300 2378634..2378762 + 129 WP_000237817.1 hypothetical protein Antitoxin
Bt4C1_RS12305 2378835..2379011 + 177 WP_063675369.1 stage II sporulation protein SB -
Bt4C1_RS12310 2379030..2379419 - 390 WP_000712655.1 YxeA family protein -
Bt4C1_RS12315 2379631..2381100 + 1470 WP_000287541.1 beta-Ala-His dipeptidase -
Bt4C1_RS12320 2381413..2382222 + 810 WP_000678376.1 C39 family peptidase -
Bt4C1_RS12325 2382248..2382853 + 606 WP_000527414.1 hypothetical protein -
Bt4C1_RS12330 2382994..2383245 - 252 WP_063675370.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2334020..2389541 55521


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28335.09 Da        Isoelectric Point: 8.2998

>T62595 WP_000624981.1 NZ_CP015176:2377784-2378521 [Bacillus thuringiensis serovar alesti]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T62595 NZ_CP015176:2377784-2378521 [Bacillus thuringiensis serovar alesti]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACCACAATTATTCAAAATGTTAATCCAGCAGTTTTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAGAGCTTAAATGTATTTTTAGATTCATATGGGGAAAAAATTGGCGCGAAAATT
GTTGTTTTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 4981.37 Da        Isoelectric Point: 3.9150

>AT62595 WP_000237817.1 NZ_CP015176:2378634-2378762 [Bacillus thuringiensis serovar alesti]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Download         Length: 129 bp

>AT62595 NZ_CP015176:2378634-2378762 [Bacillus thuringiensis serovar alesti]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATGC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6D1SWF1

Antitoxin

Source ID Structure
AlphaFold DB B7IX39

References