62594

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 220750..221392 Replicon chromosome
Accession NZ_CP015176
Organism Bacillus thuringiensis serovar alesti strain BGSC 4C1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag Bt4C1_RS01230 Protein ID WP_000635965.1
Coordinates 221042..221392 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag Bt4C1_RS01225 Protein ID WP_000004570.1
Coordinates 220750..221037 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Bt4C1_RS01200 216067..217029 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
Bt4C1_RS01205 217022..217594 - 573 WP_000908529.1 rhomboid family intramembrane serine protease -
Bt4C1_RS01210 217688..218047 + 360 WP_000583420.1 holo-ACP synthase -
Bt4C1_RS01215 218204..219154 + 951 WP_002084009.1 outer membrane lipoprotein carrier protein LolA -
Bt4C1_RS01220 219272..220441 + 1170 WP_000390595.1 alanine racemase -
Bt4C1_RS01225 220750..221037 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
Bt4C1_RS01230 221042..221392 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
Bt4C1_RS01235 221460..223628 + 2169 WP_000426209.1 RNA-binding transcriptional accessory protein -
Bt4C1_RS29555 223686..223802 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
Bt4C1_RS01240 223998..224456 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T62594 WP_000635965.1 NZ_CP015176:221042-221392 [Bacillus thuringiensis serovar alesti]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T62594 NZ_CP015176:221042-221392 [Bacillus thuringiensis serovar alesti]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT62594 WP_000004570.1 NZ_CP015176:220750-221037 [Bacillus thuringiensis serovar alesti]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT62594 NZ_CP015176:220750-221037 [Bacillus thuringiensis serovar alesti]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References