Detailed information of TA system TA062579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 6837..7366
• Replicon: chromosome
• Accession: NZ_CP015173
• Organism: Staphylococcus aureus strain RIVM6519

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A5J11_RS00045
• Protein ID: WP_000621175.1
• Coordinates: 6837..7199 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: A5J11_RS00050
• Protein ID: WP_000948331.1
• Coordinates: 7196..7366 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5J11_RS00020	3816..4586	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
A5J11_RS00025	4561..5040	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
A5J11_RS00030	5042..5368	-	327	WP_001052491.1	anti-sigma factor antagonist	-
A5J11_RS00035	5487..6488	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
A5J11_RS00045	6837..7199	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A5J11_RS00050	7196..7366	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A5J11_RS00055	7451..8599	-	1149	WP_001281154.1	alanine racemase	-
A5J11_RS00060	8665..9024	-	360	WP_000581197.1	holo-ACP synthase	-
A5J11_RS00065	9028..9519	-	492	WP_001205912.1	PH domain-containing protein	-
A5J11_RS00070	9506..11089	-	1584	WP_001294626.1	PH domain-containing protein	-
A5J11_RS00075	11082..11561	-	480	WP_001287087.1	hypothetical protein	-
A5J11_RS00080	11769..12329	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T62579 WP_000621175.1 NZ_CP015173:c7199-6837 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T62579 NZ_CP015173:c7199-6837 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT62579 WP_000948331.1 NZ_CP015173:c7366-7196 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT62579 NZ_CP015173:c7366-7196 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1