Detailed information of TA system TA062577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 46785..47413
• Replicon: plasmid unnamed2
• Accession: NZ_CP015170
• Organism: Acetobacter ascendens strain LMG 1591

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A4R89_RS15380
• Protein ID: WP_070325489.1
• Coordinates: 47009..47413 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7JJ36
• Locus tag: A4R89_RS15375
• Protein ID: WP_012813340.1
• Coordinates: 46785..47009 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R89_RS15355	42917..43597	+	681	Protein_45	IS110 family transposase	-
A4R89_RS15360	43879..44718	-	840	Protein_46	IS5 family transposase	-
A4R89_RS15365	44797..45123	+	327	Protein_47	hypothetical protein	-
A4R89_RS15370	45246..46679	+	1434	WP_070325593.1	hypothetical protein	-
A4R89_RS15375	46785..47009	+	225	WP_012813340.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A4R89_RS15380	47009..47413	+	405	WP_070325489.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A4R89_RS16745	47608..47808	+	201	WP_012813343.1	type II toxin-antitoxin system CcdA family antitoxin	-
A4R89_RS15385	47809..48114	+	306	WP_042788970.1	CcdB family protein	-
A4R89_RS15390	48394..49008	-	615	WP_070325618.1	recombinase family protein	-
A4R89_RS15395	49134..51311	+	2178	Protein_54	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..117661	117661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15180.32 Da
• Isoelectric Point: 6.0964

>T62577 WP_070325489.1 NZ_CP015170:47009-47413 [Acetobacter ascendens]
MLRYLLDTNFCIRVLRDRPQGLRERFNDNAEALCISDVVLYELLYGAEKSHDPAKIRREVEHFAARLSVLPFDDDAAAHT
AEIRVELEKRGCVIGPYDLMIAGHARSRGLVVVTGNLRELTRVDGLRSEDWTAG

Nucleotide

• Length: 405 bp

>T62577 NZ_CP015170:47009-47413 [Acetobacter ascendens]
ATGCTTCGTTATCTTCTGGATACAAACTTTTGCATTCGTGTCCTGCGTGATCGTCCGCAAGGCTTGCGTGAGCGTTTCAA
TGATAACGCCGAGGCGCTCTGCATCTCGGATGTCGTGCTTTACGAACTTCTCTATGGAGCCGAAAAATCTCACGATCCGG
CAAAGATTCGACGTGAGGTTGAGCATTTTGCCGCACGTCTGTCTGTTTTGCCGTTTGATGATGACGCCGCAGCCCATACA
GCGGAAATCAGGGTAGAGCTGGAAAAGCGTGGCTGTGTCATCGGCCCATACGATTTGATGATTGCCGGGCACGCTCGAAG
CAGGGGGCTTGTCGTTGTGACTGGAAACTTACGAGAACTTACGAGGGTAGATGGGTTGCGATCTGAGGATTGGACGGCCG
GTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8310.55 Da
• Isoelectric Point: 8.9793

>AT62577 WP_012813340.1 NZ_CP015170:46785-47009 [Acetobacter ascendens]
MLTRTTLFLSNRSQAVRLPKAVAFDVNLKDVVIIPDGARRIIAPANAAWDDFFAAPGTPLPERDQPRMQERESF

Nucleotide

• Length: 225 bp

>AT62577 NZ_CP015170:46785-47009 [Acetobacter ascendens]
ATGCTCACCCGCACGACGCTCTTTCTGTCCAATCGCTCCCAGGCCGTGCGTCTTCCCAAAGCGGTTGCATTTGACGTCAA
CCTGAAAGACGTCGTGATCATCCCGGACGGTGCGCGTCGTATAATCGCACCTGCAAATGCCGCATGGGACGATTTCTTTG
CCGCGCCGGGCACTCCACTGCCTGAGCGTGATCAGCCGAGGATGCAGGAACGCGAAAGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A511FS67