Detailed information of TA system TA062576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 172464..173092
• Replicon: plasmid unnamed1
• Accession: NZ_CP015169
• Organism: Acetobacter ascendens strain LMG 1591

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A4R89_RS14650
• Protein ID: WP_070325489.1
• Coordinates: 172688..173092 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7JJ36
• Locus tag: A4R89_RS14645
• Protein ID: WP_012813340.1
• Coordinates: 172464..172688 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R89_RS14620	167578..168963	+	1386	WP_070325487.1	IS1380 family transposase	-
A4R89_RS17265	169387..170058	-	672	WP_082247084.1	IS3 family transposase	-
A4R89_RS17270	170055..170324	-	270	WP_070325488.1	transposase	-
A4R89_RS14635	170392..170802	+	411	Protein_166	hypothetical protein	-
A4R89_RS14640	170925..172358	+	1434	WP_029604385.1	hypothetical protein	-
A4R89_RS14645	172464..172688	+	225	WP_012813340.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A4R89_RS14650	172688..173092	+	405	WP_070325489.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A4R89_RS16640	173300..173386	+	87	Protein_170	type II toxin-antitoxin system CcdA family antitoxin	-
A4R89_RS14655	173482..174867	+	1386	WP_070324433.1	IS1380 family transposase	-
A4R89_RS16645	174999..175169	+	171	WP_082247085.1	type II toxin-antitoxin system CcdA family antitoxin	-
A4R89_RS14660	175170..175475	+	306	WP_012813344.1	CcdB family protein	-
A4R89_RS14665	175608..176441	-	834	WP_070325490.1	TIGR02391 family protein	-
A4R89_RS14670	176410..177267	-	858	WP_070325491.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..259464	259464
	-	inside	IScluster/Tn	-	-	161233..174867	13634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15180.32 Da
• Isoelectric Point: 6.0964

>T62576 WP_070325489.1 NZ_CP015169:172688-173092 [Acetobacter ascendens]
MLRYLLDTNFCIRVLRDRPQGLRERFNDNAEALCISDVVLYELLYGAEKSHDPAKIRREVEHFAARLSVLPFDDDAAAHT
AEIRVELEKRGCVIGPYDLMIAGHARSRGLVVVTGNLRELTRVDGLRSEDWTAG

Nucleotide

• Length: 405 bp

>T62576 NZ_CP015169:172688-173092 [Acetobacter ascendens]
ATGCTTCGTTATCTTCTGGATACAAACTTTTGCATTCGTGTCCTGCGTGATCGTCCGCAAGGCTTGCGTGAGCGTTTCAA
TGATAACGCCGAGGCGCTCTGCATCTCGGATGTCGTGCTTTACGAACTTCTCTATGGAGCCGAAAAATCTCACGATCCGG
CAAAGATTCGACGTGAGGTTGAGCATTTTGCCGCACGTCTGTCTGTTTTGCCGTTTGATGATGACGCCGCAGCCCATACA
GCGGAAATCAGGGTAGAGCTGGAAAAGCGTGGCTGTGTCATCGGCCCATACGATTTGATGATTGCCGGGCACGCTCGAAG
CAGGGGGCTTGTCGTTGTGACTGGAAACTTACGAGAACTTACGAGGGTAGATGGGTTGCGATCTGAGGATTGGACGGCCG
GTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8310.55 Da
• Isoelectric Point: 8.9793

>AT62576 WP_012813340.1 NZ_CP015169:172464-172688 [Acetobacter ascendens]
MLTRTTLFLSNRSQAVRLPKAVAFDVNLKDVVIIPDGARRIIAPANAAWDDFFAAPGTPLPERDQPRMQERESF

Nucleotide

• Length: 225 bp

>AT62576 NZ_CP015169:172464-172688 [Acetobacter ascendens]
ATGCTCACCCGCACGACGCTCTTTCTGTCCAATCGCTCCCAGGCCGTGCGTCTTCCCAAAGCGGTTGCATTTGACGTCAA
CCTGAAAGACGTCGTGATCATCCCGGACGGTGCGCGTCGTATAATCGCACCTGCAAATGCCGCATGGGACGATTTCTTTG
CCGCGCCGGGCACTCCACTGCCTGAGCGTGATCAGCCGAGGATGCAGGAACGCGAAAGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A511FS67