Detailed information of TA system TA062575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 23723..24393
• Replicon: plasmid unnamed1
• Accession: NZ_CP015169
• Organism: Acetobacter ascendens strain LMG 1591

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A4R89_RS13955
• Protein ID: WP_070325422.1
• Coordinates: 23723..24145 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A401X711
• Locus tag: A4R89_RS13960
• Protein ID: WP_062144457.1
• Coordinates: 24142..24393 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R89_RS13930	18762..19520	+	759	WP_082247074.1	Bro-N domain-containing protein	-
A4R89_RS13935	19616..19894	+	279	WP_070325418.1	HU family DNA-binding protein	-
A4R89_RS16560	19973..20191	+	219	WP_170315285.1	WGR domain-containing protein	-
A4R89_RS13940	20297..20929	-	633	WP_070325419.1	recombinase family protein	-
A4R89_RS13945	21260..22966	+	1707	WP_070325420.1	Hint domain-containing protein	-
A4R89_RS13950	23052..23519	-	468	WP_081894107.1	hypothetical protein	-
A4R89_RS13955	23723..24145	-	423	WP_070325422.1	type II toxin-antitoxin system VapC family toxin	Toxin
A4R89_RS13960	24142..24393	-	252	WP_062144457.1	hypothetical protein	Antitoxin
A4R89_RS13965	24459..27563	-	3105	WP_070325423.1	Ti-type conjugative transfer relaxase TraA	-
A4R89_RS13970	27660..28019	+	360	WP_211274218.1	conjugal transfer protein TraD	-
A4R89_RS13975	28084..28338	+	255	WP_070325553.1	conjugal transfer protein TraD	-
A4R89_RS13980	28328..28702	+	375	WP_070325424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..259464	259464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15331.68 Da
• Isoelectric Point: 6.4755

>T62575 WP_070325422.1 NZ_CP015169:c24145-23723 [Acetobacter ascendens]
MILLDTNVISEPWKPVPEPRVLAWIDRQAIETLFLSAVTVAELRFGIGAMPAGRRRSVLHERLEQQILPLFAGRVLSFDL
AASQAYAELMVRARSEGRAIGKADGYIAATAASHGYAVASRDMSPFEAAGVRVLNPWEDV

Nucleotide

• Length: 423 bp

>T62575 NZ_CP015169:c24145-23723 [Acetobacter ascendens]
ATGATCCTGCTCGATACCAATGTGATCTCAGAGCCGTGGAAGCCTGTCCCGGAGCCGCGTGTTCTGGCCTGGATCGATCG
ACAGGCGATCGAGACGCTTTTCCTGTCGGCCGTCACGGTGGCGGAGCTGCGTTTCGGGATCGGGGCGATGCCGGCCGGTC
GGAGGCGTTCGGTGCTTCATGAGCGGCTGGAGCAGCAGATTCTACCTCTGTTCGCGGGGCGTGTCCTGTCGTTCGATCTG
GCGGCGTCCCAGGCTTATGCCGAGTTGATGGTGCGGGCCAGGTCTGAGGGACGGGCAATCGGCAAGGCGGATGGCTATAT
TGCGGCGACCGCGGCATCCCATGGTTATGCTGTGGCCAGTCGCGACATGTCGCCTTTCGAGGCGGCCGGTGTGCGGGTTC
TTAATCCGTGGGAGGATGTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9174.49 Da
• Isoelectric Point: 7.2058

>AT62575 WP_062144457.1 NZ_CP015169:c24393-24142 [Acetobacter ascendens]
MSSVVIRNLPEETHRALKAQAMLHGRSTEAEIRTILEAAVRPPQRVRLGSLLASIGREAGVREQDVTALQVRDQTPAEPM
SFE

Nucleotide

• Length: 252 bp

>AT62575 NZ_CP015169:c24393-24142 [Acetobacter ascendens]
ATGTCCTCTGTGGTTATTCGCAATCTTCCGGAAGAGACACATCGGGCGCTGAAGGCGCAGGCCATGCTGCATGGGCGCAG
CACGGAAGCGGAGATCCGGACCATTCTGGAAGCGGCCGTCCGGCCGCCGCAGCGGGTGCGGTTGGGCTCGCTGCTGGCTT
CCATCGGTCGAGAGGCCGGTGTGCGTGAACAGGATGTCACGGCCTTGCAGGTTCGCGATCAGACCCCGGCCGAGCCGATG
TCCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A401X711