Detailed information of TA system TA062574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 11725..12404
• Replicon: plasmid unnamed1
• Accession: NZ_CP015169
• Organism: Acetobacter ascendens strain LMG 1591

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A401X8K4
• Locus tag: A4R89_RS13885
• Protein ID: WP_042788834.1
• Coordinates: 11994..12404 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A4R89_RS13880
• Protein ID: WP_211274216.1
• Coordinates: 11725..11997 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R89_RS13855	6900..8285	+	1386	WP_070325411.1	IS1380 family transposase	-
A4R89_RS13860	8778..9458	+	681	WP_070325412.1	hypothetical protein	-
A4R89_RS13865	10253..10741	+	489	WP_070325413.1	single-stranded DNA-binding protein	-
A4R89_RS13870	10804..11241	+	438	WP_070325414.1	plasmid protein	-
A4R89_RS13875	11238..11654	+	417	WP_070325415.1	hypothetical protein	-
A4R89_RS13880	11725..11997	+	273	WP_211274216.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A4R89_RS13885	11994..12404	+	411	WP_042788834.1	type II toxin-antitoxin system VapC family toxin	Toxin
A4R89_RS16555	12752..12961	+	210	WP_082247097.1	hypothetical protein	-
A4R89_RS13895	13211..13564	-	354	WP_081273974.1	hypothetical protein	-
A4R89_RS13900	13624..14427	-	804	WP_070325416.1	hypothetical protein	-
A4R89_RS13905	14524..14805	-	282	Protein_19	hypothetical protein	-
A4R89_RS13910	14899..16284	+	1386	WP_070324433.1	IS1380 family transposase	-
A4R89_RS13915	16469..16657	-	189	Protein_21	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..259464	259464
	-	flank	IS/Tn	-	-	14899..16284	1385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14843.13 Da
• Isoelectric Point: 4.7037

>T62574 WP_042788834.1 NZ_CP015169:11994-12404 [Acetobacter ascendens]
VSTYLLDTNIISDLVRNPSGLCAQRIELIDPGDLCTSIIVAAELRYGCAKKGSAKLLNRVESVLAFIPVLPLDIPADGEY
GGIRAELEAAGQTIGMNDLLIAAHAYTLKATLVTNNTREFMRIRGLRLENWLENET

Nucleotide

• Length: 411 bp

>T62574 NZ_CP015169:11994-12404 [Acetobacter ascendens]
GTGAGCACCTATCTTCTTGATACCAACATCATCAGTGATCTGGTTCGCAATCCTTCCGGCTTATGTGCGCAACGCATTGA
GCTTATCGACCCCGGCGACCTTTGCACCAGCATCATTGTGGCTGCTGAGTTGCGTTATGGATGCGCAAAAAAAGGCTCTG
CGAAGCTATTGAACCGGGTTGAGAGCGTGCTGGCTTTTATCCCCGTCCTGCCACTCGATATTCCTGCTGACGGTGAATAC
GGTGGCATCCGTGCCGAACTCGAAGCCGCAGGGCAGACGATCGGCATGAATGATCTGCTCATCGCGGCGCATGCCTACAC
CCTCAAGGCGACCCTGGTGACGAACAACACACGTGAGTTTATGCGTATCAGAGGCTTGAGGCTCGAAAATTGGCTGGAGA
ATGAGACGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10467.11 Da
• Isoelectric Point: 4.8723

>AT62574 WP_211274216.1 NZ_CP015169:11725-11997 [Acetobacter ascendens]
MPVPKVTPRPREVKLFWNNRSQAVRIPVEFQMPGDRVLIHRDGEKLVLEPVKTPSTLKELLMAWREEPQLRPEDDFPDIQ
DVAATPEDIL

Nucleotide

• Length: 273 bp

>AT62574 NZ_CP015169:11725-11997 [Acetobacter ascendens]
ATGCCAGTTCCTAAAGTGACTCCCCGACCACGCGAAGTAAAACTGTTCTGGAACAACCGCAGTCAGGCAGTGCGGATTCC
GGTTGAATTTCAAATGCCAGGAGACCGGGTGCTGATCCACCGTGACGGTGAGAAACTCGTTCTGGAGCCAGTGAAAACGC
CTTCTACGCTGAAGGAGCTTCTCATGGCATGGCGTGAAGAGCCGCAGCTCAGGCCCGAAGATGATTTTCCTGATATTCAG
GACGTTGCCGCCACACCGGAAGATATTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A401X8K4