Detailed information of TA system TA062573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2682998..2683581
• Replicon: chromosome
• Accession: NZ_CP015168
• Organism: Acetobacter ascendens strain LMG 1591

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: F1YT37
• Locus tag: A4R89_RS13195
• Protein ID: WP_006116155.1
• Coordinates: 2683327..2683581 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A4R89_RS13190
• Protein ID: WP_070325281.1
• Coordinates: 2682998..2683330 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R89_RS13155	2678967..2679290	-	324	WP_070325278.1	SANT/Myb domain-containing protein	-
A4R89_RS13160	2679287..2679538	-	252	WP_070325279.1	hypothetical protein	-
A4R89_RS13165	2679535..2679726	-	192	WP_012812727.1	hypothetical protein	-
A4R89_RS16525	2679812..2680569	-	758	Protein_2503	IS5 family transposase	-
A4R89_RS13180	2680679..2681929	+	1251	WP_070324420.1	IS701 family transposase	-
A4R89_RS13185	2682684..2682959	+	276	WP_157886594.1	hypothetical protein	-
A4R89_RS13190	2682998..2683330	-	333	WP_070325281.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A4R89_RS13195	2683327..2683581	-	255	WP_006116155.1	type II toxin-antitoxin system HicA family toxin	Toxin
A4R89_RS13200	2683821..2684660	-	840	Protein_2508	site-specific integrase	-
A4R89_RS13215	2685822..2686541	-	720	WP_157886595.1	hypothetical protein	-
A4R89_RS13220	2686761..2688314	+	1554	Protein_2511	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2680222..2689811	9589
	-	inside	IScluster/Tn	-	-	2677022..2685787	8765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (56-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9479.92 Da
• Isoelectric Point: 10.3861

>T62573 WP_006116155.1 NZ_CP015168:c2683581-2683327 [Acetobacter ascendens]
MKAKHRRTLELIFKRPVSGNVPWADIEALFVSLGAEVSEREGSRIGVFLFDEVKVFHRPHPKPDTDKGAVASVRKWLEQN
GVTP

Nucleotide

• Length: 255 bp

>T62573 NZ_CP015168:c2683581-2683327 [Acetobacter ascendens]
ATGAAAGCCAAGCACCGTCGCACATTGGAACTGATTTTCAAGCGTCCTGTTTCGGGCAATGTGCCATGGGCAGACATTGA
AGCTTTGTTTGTTTCTCTTGGGGCGGAGGTGTCTGAAAGAGAAGGCTCCCGCATAGGGGTGTTCCTGTTTGATGAGGTGA
AGGTGTTTCACAGACCTCACCCCAAACCGGATACCGACAAGGGCGCTGTAGCCTCTGTACGAAAATGGCTTGAGCAGAAT
GGAGTGACGCCATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11933.57 Da
• Isoelectric Point: 4.8380

>AT62573 WP_070325281.1 NZ_CP015168:c2683330-2682998 [Acetobacter ascendens]
MNNVMEIGGHRAVIQFDPEIGMFRGEFLGLNGGADFYADSVAGLQQEGEASLRVFMEMCAEKGIDPVKHYSGRFVVRLPE
NVHARAVEIAAARGVSLNRLVQDALAQVDG

Nucleotide

• Length: 333 bp

>AT62573 NZ_CP015168:c2683330-2682998 [Acetobacter ascendens]
ATGAACAACGTAATGGAAATTGGCGGCCATCGGGCTGTTATTCAGTTTGACCCTGAAATCGGGATGTTTCGGGGCGAGTT
TCTGGGACTGAATGGAGGGGCTGATTTCTACGCAGATAGTGTGGCTGGCCTTCAGCAGGAAGGGGAAGCTTCCCTCCGTG
TATTCATGGAGATGTGTGCCGAAAAGGGCATTGATCCTGTGAAGCATTATTCAGGCCGGTTTGTGGTGAGGTTACCAGAA
AACGTTCATGCGCGGGCAGTCGAGATTGCAGCAGCGCGTGGTGTCAGTCTTAACCGACTGGTGCAGGATGCTCTGGCGCA
GGTTGACGGATAA

Structures

Toxin

• AlphaFold DB: A0A401X5W6