Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 14131..14741 Replicon plasmid unnamed1
Accession NZ_CP015165
Organism Acetobacter ascendens strain LMG 1590

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D8R058
Locus tag A4S02_RS14000 Protein ID WP_070324298.1
Coordinates 14328..14741 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D8R034
Locus tag A4S02_RS13995 Protein ID WP_070324297.1
Coordinates 14131..14328 (+) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4S02_RS13980 9210..10469 + 1260 WP_082246881.1 restriction endonuclease subunit S -
A4S02_RS13985 10466..13645 + 3180 WP_070324295.1 type I restriction endonuclease subunit R -
A4S02_RS13990 13662..14060 - 399 WP_070324296.1 hypothetical protein -
A4S02_RS13995 14131..14328 + 198 WP_070324297.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A4S02_RS14000 14328..14741 + 414 WP_070324298.1 PIN domain-containing protein Toxin
A4S02_RS14005 14992..15696 + 705 WP_070324299.1 hypothetical protein -
A4S02_RS14010 15736..16560 + 825 WP_082246882.1 IS5 family transposase -
A4S02_RS14015 16587..17489 + 903 WP_070324301.1 tyrosine-type recombinase/integrase -
A4S02_RS14020 17486..18151 + 666 WP_070324302.1 hypothetical protein -
A4S02_RS14025 18283..18975 + 693 WP_070324303.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49380 49380


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-115)

Antitoxin

SpoVT-AbrB domain

(5-31)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15464.86 Da        Isoelectric Point: 4.4585

>T62572 WP_070324298.1 NZ_CP015165:14328-14741 [Acetobacter ascendens]
MSLFLDTNILLYAMMEEGTEKAGIADSILLHQDCHISVQTLNEATNTLRRKTKLPLDDIKTLISDFRGLVTIHPITETVY
SRGWAIIERYGLQTYDAMILACAIENGIETLLSEDMQNGQKIFDMVTIINPFQKAID

Download         Length: 414 bp

>T62572 NZ_CP015165:14328-14741 [Acetobacter ascendens]
ATGTCACTCTTTCTGGATACGAACATCCTTCTCTATGCCATGATGGAAGAAGGAACAGAAAAGGCTGGTATTGCTGACAG
TATCCTTCTGCATCAGGACTGCCATATCTCGGTGCAGACACTCAACGAAGCAACCAATACGCTAAGACGCAAAACTAAAC
TTCCGCTAGACGACATCAAAACCCTTATCTCGGATTTCCGGGGACTGGTCACAATCCACCCCATCACAGAAACCGTTTAC
AGTCGGGGCTGGGCCATCATTGAACGCTATGGACTCCAGACTTACGACGCCATGATTCTGGCCTGCGCCATAGAAAACGG
TATCGAAACCCTATTGTCCGAAGACATGCAGAATGGTCAGAAGATTTTTGATATGGTGACAATCATTAACCCCTTTCAGA
AAGCCATAGATTAG

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7382.56 Da        Isoelectric Point: 10.7798

>AT62572 WP_070324297.1 NZ_CP015165:14131-14328 [Acetobacter ascendens]
MQVSKWGNSLAVRIPSHIVKQLGLQEGDNVEALFTRLKSKEEALRSLKEIGKKLPSGFRFERSKD

Download         Length: 198 bp

>AT62572 NZ_CP015165:14131-14328 [Acetobacter ascendens]
ATGCAAGTGTCCAAGTGGGGTAACAGTCTGGCTGTCCGAATTCCCTCGCATATTGTCAAACAACTGGGTCTTCAGGAAGG
TGACAATGTGGAAGCTCTTTTTACACGCCTCAAATCCAAAGAAGAAGCTCTTCGTTCCTTGAAAGAAATCGGAAAGAAAC
TGCCTTCCGGCTTTCGGTTTGAACGCTCAAAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8R058

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8R034

References