Detailed information of TA system TA062571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1060822..1061450
• Replicon: chromosome
• Accession: NZ_CP015164
• Organism: Acetobacter ascendens strain LMG 1590

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A4S02_RS05135
• Protein ID: WP_003630720.1
• Coordinates: 1060822..1061226 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7JJ36
• Locus tag: A4S02_RS05140
• Protein ID: WP_012813340.1
• Coordinates: 1061226..1061450 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4S02_RS14890	1056031..1056979	-	949	Protein_994	IS630 family transposase	-
A4S02_RS05110	1057343..1058027	-	685	Protein_995	DUF1275 domain-containing protein	-
A4S02_RS05115	1058027..1059289	-	1263	WP_070323151.1	sodium:proton antiporter	-
A4S02_RS05120	1059421..1059714	-	294	WP_070323152.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A4S02_RS05125	1059711..1059971	-	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
A4S02_RS05130	1060121..1060426	-	306	WP_012813344.1	CcdB family protein	-
A4S02_RS14895	1060427..1060627	-	201	WP_012813343.1	type II toxin-antitoxin system CcdA family antitoxin	-
A4S02_RS05135	1060822..1061226	-	405	WP_003630720.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A4S02_RS05140	1061226..1061450	-	225	WP_012813340.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A4S02_RS05145	1061556..1062989	-	1434	WP_070323153.1	hypothetical protein	-
A4S02_RS15600	1063001..1063498	+	498	WP_157885528.1	hypothetical protein	-
A4S02_RS05155	1063514..1064596	+	1083	WP_208858931.1	IS5 family transposase	-
A4S02_RS05160	1064713..1065048	+	336	Protein_1006	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1050591..1073592	23001
	-	flank	IS/Tn	-	-	1064767..1065048	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15152.26 Da
• Isoelectric Point: 6.0964

>T62571 WP_003630720.1 NZ_CP015164:c1061226-1060822 [Acetobacter ascendens]
MLRYLLDTNFCIRVLRDRPQGLRERFNDNAEALCISDVVLYELLYGAEKSHDPAKIRREVEHFAARLSVLPFDDDAAAHT
AEIRAELEKRGCVIGPYDLMIAGHARSRGLVVVTGNLRELTRVDGLRSEDWTAG

Nucleotide

• Length: 405 bp

>T62571 NZ_CP015164:c1061226-1060822 [Acetobacter ascendens]
ATGCTTCGTTATCTTCTGGATACAAACTTTTGCATTCGTGTCCTGCGTGATCGTCCGCAAGGCTTGCGTGAGCGTTTCAA
TGATAACGCCGAGGCGCTCTGCATCTCGGATGTCGTGCTTTACGAACTTCTCTATGGAGCCGAAAAATCTCACGATCCGG
CAAAGATTCGACGTGAGGTTGAGCATTTTGCCGCACGTCTGTCTGTTTTGCCGTTTGATGATGACGCCGCAGCCCATACA
GCGGAAATCAGGGCAGAGCTGGAAAAGCGTGGCTGTGTCATCGGCCCATACGATTTGATGATTGCCGGGCACGCTCGAAG
CAGGGGGCTTGTCGTTGTGACTGGAAACTTACGAGAACTTACGAGGGTAGATGGGTTGCGATCTGAGGATTGGACGGCCG
GTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8310.55 Da
• Isoelectric Point: 8.9793

>AT62571 WP_012813340.1 NZ_CP015164:c1061450-1061226 [Acetobacter ascendens]
MLTRTTLFLSNRSQAVRLPKAVAFDVNLKDVVIIPDGARRIIAPANAAWDDFFAAPGTPLPERDQPRMQERESF

Nucleotide

• Length: 225 bp

>AT62571 NZ_CP015164:c1061450-1061226 [Acetobacter ascendens]
ATGCTCACCCGCACGACGCTCTTTCTGTCCAATCGCTCCCAGGCCGTGCGTCTTCCCAAAGCGGTTGCATTTGACGTCAA
CCTGAAAGACGTCGTGATCATCCCGGACGGTGCGCGTCGTATAATCGCACCTGCAAATGCCGCATGGGACGATTTCTTTG
CCGCGCCGGGCACTCCACTGCCTGAGCGTGATCAGCCGAGGATGCAGGAACGCGAAAGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A511FS67