Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 59198..59757 Replicon plasmid pECO-93a
Accession NZ_CP015161
Organism Escherichia coli strain Eco889

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag WM90_RS26565 Protein ID WP_000421257.1
Coordinates 59198..59473 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A827YCB8
Locus tag WM90_RS26570 Protein ID WP_024186511.1
Coordinates 59473..59757 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM90_RS26535 55421..56185 - 765 WP_064847776.1 helix-turn-helix domain-containing protein -
WM90_RS26545 57190..57792 + 603 WP_064847778.1 proQ/FINO family protein -
WM90_RS26550 57848..58348 + 501 WP_024230392.1 hypothetical protein -
WM90_RS30740 58353..58619 + 267 WP_021576094.1 hypothetical protein -
WM90_RS26560 58811..59092 - 282 WP_064847779.1 hypothetical protein -
WM90_RS26565 59198..59473 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
WM90_RS26570 59473..59757 - 285 WP_024186511.1 ribbon-helix-helix domain-containing protein Antitoxin
WM90_RS26575 60662..61534 - 873 WP_064772923.1 incFII family plasmid replication initiator RepA -
WM90_RS26585 62511..62774 + 264 WP_001387472.1 ash family protein -
WM90_RS26590 62692..62979 + 288 WP_033548823.1 conjugal transfer protein TraA -
WM90_RS26595 63421..63954 + 534 WP_033548834.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88023 88023


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T62566 WP_000421257.1 NZ_CP015161:c59473-59198 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T62566 NZ_CP015161:c59473-59198 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10502.95 Da        Isoelectric Point: 5.6558

>AT62566 WP_024186511.1 NZ_CP015161:c59757-59473 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT62566 NZ_CP015161:c59757-59473 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A827YCB8

References