Detailed information of TA system TA062547

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4757903..4758737
• Replicon: chromosome
• Accession: NZ_CP015159
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: WM90_RS23655
• Protein ID: WP_001546109.1
• Coordinates: 4758360..4758737 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7I0L0C8
• Locus tag: WM90_RS23650
• Protein ID: WP_001546108.1
• Coordinates: 4757903..4758283 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS23615	4754183..4754638	+	456	WP_001545736.1	hypothetical protein	-
WM90_RS23620	4754717..4754950	+	234	WP_001119729.1	DUF905 family protein	-
WM90_RS23625	4755050..4755868	+	819	WP_001234620.1	DUF945 domain-containing protein	-
WM90_RS23630	4755923..4756408	+	486	WP_000849588.1	antirestriction protein	-
WM90_RS23635	4756424..4756900	+	477	WP_042962214.1	RadC family protein	-
WM90_RS23640	4756969..4757190	+	222	WP_000692345.1	DUF987 domain-containing protein	-
WM90_RS23645	4757209..4757853	+	645	WP_000086755.1	hypothetical protein	-
WM90_RS23650	4757903..4758283	+	381	WP_001546108.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM90_RS23655	4758360..4758737	+	378	WP_001546109.1	TA system toxin CbtA family protein	Toxin
WM90_RS23660	4758734..4759221	+	488	Protein_4494	hypothetical protein	-
WM90_RS23665	4759238..4759435	+	198	WP_000839260.1	DUF957 domain-containing protein	-
WM90_RS23670	4759520..4760365	+	846	WP_001280441.1	DUF4942 domain-containing protein	-
WM90_RS23675	4760434..4760829	+	396	WP_000208383.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
WM90_RS23680	4760822..4761756	+	935	Protein_4498	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
WM90_RS30485	4762188..4762343	+	156	WP_000729639.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.00 Da
• Isoelectric Point: 8.5190

>T62547 WP_001546109.1 NZ_CP015159:4758360-4758737 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYKMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T62547 NZ_CP015159:4758360-4758737 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13997.78 Da
• Isoelectric Point: 5.0823

>AT62547 WP_001546108.1 NZ_CP015159:4757903-4758283 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT62547 NZ_CP015159:4757903-4758283 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A7I0L0C8