Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2034569..2035187 Replicon chromosome
Accession NZ_CP015159
Organism Escherichia coli strain Eco889

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag WM90_RS09985 Protein ID WP_001291435.1
Coordinates 2034569..2034787 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag WM90_RS09990 Protein ID WP_000344800.1
Coordinates 2034813..2035187 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM90_RS09950 2029857..2030429 + 573 WP_000779841.1 YbaY family lipoprotein -
WM90_RS09955 2030460..2030771 - 312 WP_000409908.1 MGMT family protein -
WM90_RS09965 2031150..2031503 + 354 WP_000878147.1 DUF1428 family protein -
WM90_RS09970 2031545..2033095 - 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
WM90_RS09975 2033259..2033729 - 471 WP_000136192.1 YlaC family protein -
WM90_RS09980 2033845..2034396 - 552 WP_000102539.1 maltose O-acetyltransferase -
WM90_RS09985 2034569..2034787 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
WM90_RS09990 2034813..2035187 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
WM90_RS09995 2035733..2038882 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
WM90_RS10000 2038905..2040098 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T62529 WP_001291435.1 NZ_CP015159:c2034787-2034569 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T62529 NZ_CP015159:c2034787-2034569 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT62529 WP_000344800.1 NZ_CP015159:c2035187-2034813 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT62529 NZ_CP015159:c2035187-2034813 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References