Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1621813..1622363 Replicon chromosome
Accession NZ_CP015159
Organism Escherichia coli strain Eco889

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag WM90_RS08075 Protein ID WP_001160966.1
Coordinates 1622049..1622363 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag WM90_RS08070 Protein ID WP_000125566.1
Coordinates 1621813..1622046 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM90_RS08045 1616886..1617173 + 288 WP_000203741.1 ferredoxin-like protein FixX -
WM90_RS08050 1617232..1618563 + 1332 WP_001183207.1 MFS transporter -
WM90_RS08055 1618671..1619201 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
WM90_RS08060 1619194..1621056 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
WM90_RS08065 1621248..1621727 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
WM90_RS08070 1621813..1622046 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
WM90_RS08075 1622049..1622363 + 315 WP_001160966.1 CcdB family protein Toxin
WM90_RS08080 1622360..1623208 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
WM90_RS08085 1623215..1623592 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
WM90_RS08090 1623595..1624416 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
WM90_RS08095 1624413..1625402 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
WM90_RS08100 1625402..1626688 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T62527 WP_001160966.1 NZ_CP015159:1622049-1622363 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T62527 NZ_CP015159:1622049-1622363 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT62527 WP_000125566.1 NZ_CP015159:1621813-1622046 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT62527 NZ_CP015159:1621813-1622046 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References