Detailed information of TA system TA062525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1472346..1473166
• Replicon: chromosome
• Accession: NZ_CP015159
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: WM90_RS07265
• Protein ID: WP_001054379.1
• Coordinates: 1472909..1473166 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: WM90_RS07260
• Protein ID: WP_000123957.1
• Coordinates: 1472346..1472897 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS07235	1468040..1469353	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
WM90_RS07240	1469365..1469643	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
WM90_RS07245	1469640..1470761	-	1122	WP_000010833.1	M42 family metallopeptidase	-
WM90_RS30590	1471006..1471122	-	117	Protein_1356	VOC family protein	-
WM90_RS07250	1471160..1471420	-	261	WP_000077645.1	hypothetical protein	-
WM90_RS07255	1471548..1472294	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
WM90_RS07260	1472346..1472897	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
WM90_RS07265	1472909..1473166	-	258	WP_001054379.1	YjhX family toxin	Toxin
WM90_RS07270	1473543..1473842	-	300	WP_001332034.1	GNAT family N-acetyltransferase	-
WM90_RS07275	1473920..1474651	+	732	Protein_1362	DNA helicase	-
WM90_RS07280	1474681..1476294	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
WM90_RS07285	1476325..1476675	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
WM90_RS07290	1476672..1477097	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
WM90_RS07295	1477183..1477686	+	504	Protein_1366	DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T62525 WP_001054379.1 NZ_CP015159:c1473166-1472909 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T62525 NZ_CP015159:c1473166-1472909 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT62525 WP_000123957.1 NZ_CP015159:c1472897-1472346 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT62525 NZ_CP015159:c1472897-1472346 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030