Detailed information of TA system TA062524

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1440963..1441797
• Replicon: chromosome
• Accession: NZ_CP015159
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: WM90_RS07115
• Protein ID: WP_000854770.1
• Coordinates: 1441420..1441797 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: WM90_RS07110
• Protein ID: WP_001280950.1
• Coordinates: 1440963..1441331 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS07070	1436115..1438016	+	1902	Protein_1322	Ag43/Cah family autotransporter adhesin	-
WM90_RS07075	1438087..1438245	+	159	WP_001323397.1	DUF905 family protein	-
WM90_RS07080	1438400..1439218	+	819	WP_001234712.1	DUF945 domain-containing protein	-
WM90_RS07090	1439560..1440024	+	465	WP_000855061.1	antirestriction protein	-
WM90_RS07095	1440040..1440516	+	477	WP_001186779.1	RadC family protein	-
WM90_RS07100	1440579..1440800	+	222	WP_001220314.1	DUF987 domain-containing protein	-
WM90_RS07110	1440963..1441331	+	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM90_RS07115	1441420..1441797	+	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
WM90_RS07120	1441794..1442282	+	489	WP_000761690.1	hypothetical protein	-
WM90_RS07125	1442299..1442475	+	177	WP_000839286.1	DUF957 domain-containing protein	-
WM90_RS30315	1442575..1442733	+	159	WP_001467148.1	hypothetical protein	-
WM90_RS29770	1443097..1443273	+	177	Protein_1333	helix-turn-helix domain-containing protein	-
WM90_RS07135	1444046..1445686	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1392955..1453443	60488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T62524 WP_000854770.1 NZ_CP015159:1441420-1441797 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T62524 NZ_CP015159:1441420-1441797 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT62524 WP_001280950.1 NZ_CP015159:1440963-1441331 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT62524 NZ_CP015159:1440963-1441331 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885