Detailed information of TA system TA062523

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1242332..1243166
• Replicon: chromosome
• Accession: NZ_CP015159
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: WM90_RS06080
• Protein ID: WP_001545731.1
• Coordinates: 1242332..1242709 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: WM90_RS06085
• Protein ID: WP_001545732.1
• Coordinates: 1242786..1243166 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS06040	1237582..1238979	+	1398	WP_000404886.1	PTS ascorbate transporter subunit IIC	-
WM90_RS06045	1238995..1239300	+	306	WP_000218362.1	PTS ascorbate transporter subunit IIB	-
WM90_RS06050	1239310..1239774	+	465	WP_000776505.1	PTS ascorbate transporter subunit IIA	-
WM90_RS06055	1239788..1240438	+	651	WP_000056749.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
WM90_RS06065	1240710..1241528	-	819	WP_001545729.1	DUF4942 domain-containing protein	-
WM90_RS06070	1241642..1241830	-	189	WP_001545730.1	DUF957 domain-containing protein	-
WM90_RS06075	1241847..1242335	-	489	WP_000761698.1	hypothetical protein	-
WM90_RS06080	1242332..1242709	-	378	WP_001545731.1	TA system toxin CbtA family protein	Toxin
WM90_RS06085	1242786..1243166	-	381	WP_001545732.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM90_RS06090	1243216..1243860	-	645	WP_001545733.1	hypothetical protein	-
WM90_RS06095	1243879..1244100	-	222	WP_000692353.1	DUF987 domain-containing protein	-
WM90_RS06100	1244163..1244639	-	477	WP_001545734.1	RadC family protein	-
WM90_RS06105	1244655..1245140	-	486	WP_000849588.1	antirestriction protein	-
WM90_RS06110	1245195..1246013	-	819	WP_001545735.1	DUF945 domain-containing protein	-
WM90_RS30560	1246113..1246322	-	210	Protein_1146	DUF905 family protein	-
WM90_RS06120	1246423..1246878	-	456	WP_001545736.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14054.12 Da
• Isoelectric Point: 9.1510

>T62523 WP_001545731.1 NZ_CP015159:c1242709-1242332 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRAIGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T62523 NZ_CP015159:c1242709-1242332 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGATCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13994.73 Da
• Isoelectric Point: 5.0696

>AT62523 WP_001545732.1 NZ_CP015159:c1243166-1242786 [Escherichia coli]
VSDTLSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT62523 NZ_CP015159:c1243166-1242786 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG