Detailed information of TA system TA062521

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 584459..585257
• Replicon: chromosome
• Accession: NZ_CP015159
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: WM90_RS02935
• Protein ID: WP_000854735.1
• Coordinates: 584880..585257 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: WM90_RS02930
• Protein ID: WP_001285415.1
• Coordinates: 584459..584833 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS02895	580390..581070	+	681	WP_001282921.1	WYL domain-containing protein	-
WM90_RS02900	581218..581895	+	678	WP_001097302.1	hypothetical protein	-
WM90_RS02905	581901..582134	+	234	WP_001278293.1	DUF905 family protein	-
WM90_RS02910	582224..583042	+	819	WP_001175149.1	DUF945 domain-containing protein	-
WM90_RS02915	583124..583603	+	480	WP_000844100.1	antirestriction protein	-
WM90_RS02920	583619..584095	+	477	WP_001366855.1	RadC family protein	-
WM90_RS02925	584158..584379	+	222	WP_000692309.1	DUF987 domain-containing protein	-
WM90_RS02930	584459..584833	+	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM90_RS02935	584880..585257	+	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
WM90_RS02940	585254..585742	+	489	WP_000761680.1	hypothetical protein	-
WM90_RS02945	585760..585957	+	198	WP_000839266.1	DUF957 domain-containing protein	-
WM90_RS02950	586054..586896	+	843	WP_000036706.1	DUF4942 domain-containing protein	-
WM90_RS02960	587492..587989	+	498	WP_000509815.1	hypothetical protein	-
WM90_RS02965	588167..589090	+	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
WM90_RS02970	589094..589912	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	552154..587989	35835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T62521 WP_000854735.1 NZ_CP015159:584880-585257 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T62521 NZ_CP015159:584880-585257 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT62521 WP_001285415.1 NZ_CP015159:584459-584833 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT62521 NZ_CP015159:584459-584833 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A067H947