Detailed information of TA system TA062515

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 5131..5873
• Replicon: plasmid pBTBC4
• Accession: NZ_CP015154
• Organism: Bacillus thuringiensis strain Bc601

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: BtBc_RS29790
• Protein ID: WP_016097319.1
• Coordinates: 5289..5873 (+)
• Length: 195 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	5131..5227 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BtBc_RS29770	682..2314	+	1633	Protein_1	hypothetical protein	-
BtBc_RS29775	2376..3362	+	987	WP_016097316.1	hypothetical protein	-
BtBc_RS29780	3384..3935	+	552	Protein_3	metal-dependent hydrolase	-
BtBc_RS29785	4015..5038	+	1024	Protein_4	DUF3991 and TOPRIM domain-containing protein	-
-	5131..5227	+	97	NuclAT_0	-	Antitoxin
-	5131..5227	+	97	NuclAT_0	-	Antitoxin
-	5131..5227	+	97	NuclAT_0	-	Antitoxin
-	5131..5227	+	97	NuclAT_0	-	Antitoxin
BtBc_RS29790	5289..5873	+	585	WP_016097319.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
BtBc_RS29795	5941..7242	+	1302	WP_016097320.1	hypothetical protein	-
BtBc_RS29800	7349..7729	+	381	WP_016097321.1	hypothetical protein	-
BtBc_RS29805	7850..9991	+	2142	WP_016097322.1	type IA DNA topoisomerase	-
BtBc_RS29810	10021..10467	+	447	WP_016097323.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82417	82417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-169)

Sequences

Toxin

Protein

• Length: 195 a.a.
• Molecular weight: 22900.17 Da
• Isoelectric Point: 8.9694

>T62515 WP_016097319.1 NZ_CP015154:5289-5873 [Bacillus thuringiensis]
MANKDNPKFYTISTEYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDTDEKRIG
VLLVNNMIPVPEKECKEIDIAEKTAADPQYGNLMLKQYLFLKENMDRVTNKVEKVYKDVTVQGKPSHKQKFLKGVCCDFP
KLEEKCQEYKERDQAKERDKARRIAYMRQMGRER

Nucleotide

• Length: 585 bp

>T62515 NZ_CP015154:5289-5873 [Bacillus thuringiensis]
TTGGCTAATAAAGATAATCCTAAATTCTACACCATTTCAACTGAATATATAGATTATTTAAGAGAAACGGATAGTAAAGT
ACCTTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGTGTATTGGAAAAAATCAATGGTCATGATTATTTTGTAC
CTTTAACATCACGTAACGATAAAAATTTTAACAGTCAGGTCAGTGTGAAATTATTCGATACTGATGAAAAGCGAATTGGA
GTTTTATTGGTTAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAACAGCAGCAGA
CCCTCAGTATGGTAATTTGATGTTAAAACAGTATTTATTTTTAAAAGAAAATATGGATCGAGTTACTAATAAAGTGGAAA
AAGTATATAAAGATGTAACTGTACAAGGAAAGCCTTCACATAAACAAAAATTCTTAAAAGGAGTTTGTTGTGATTTCCCT
AAATTAGAAGAAAAGTGCCAAGAATATAAAGAAAGAGATCAAGCTAAAGAAAGAGATAAAGCTAGAAGAATAGCATATAT
GCGTCAAATGGGGCGTGAGAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT62515 NZ_CP015154:5131-5227 [Bacillus thuringiensis]
GTAACCTTACTTTAGTAGGTGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCTAAAAAA

Structures

Antitoxin