Detailed information of TA system TA062514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2552241..2553219
• Replicon: chromosome
• Accession: NZ_CP015150
• Organism: Bacillus thuringiensis strain Bc601

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: BtBc_RS13235
• Protein ID: WP_000624977.1
• Coordinates: 2552241..2552978 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: BtBc_RS13240
• Protein ID: WP_000237818.1
• Coordinates: 2553091..2553219 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BtBc_RS13215	2547884..2548666	+	783	WP_000381800.1	class I SAM-dependent methyltransferase	-
BtBc_RS13220	2548824..2550509	-	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
BtBc_RS13225	2550617..2551099	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
BtBc_RS13230	2551266..2552003	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BtBc_RS13235	2552241..2552978	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
BtBc_RS13240	2553091..2553219	+	129	WP_000237818.1	hypothetical protein	Antitoxin
BtBc_RS13245	2553292..2553468	+	177	WP_000852629.1	stage II sporulation protein SB	-
BtBc_RS13250	2553487..2553876	-	390	WP_000713866.1	YxeA family protein	-
BtBc_RS13255	2554088..2555557	+	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
BtBc_RS13260	2555803..2556612	+	810	WP_000678509.1	C39 family peptidase	-
BtBc_RS13265	2556638..2557240	+	603	WP_000517260.1	hypothetical protein	-
BtBc_RS13270	2557480..2557731	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T62514 WP_000624977.1 NZ_CP015150:2552241-2552978 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T62514 NZ_CP015150:2552241-2552978 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT62514 WP_000237818.1 NZ_CP015150:2553091-2553219 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT62514 NZ_CP015150:2553091-2553219 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5