Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239171..239813 Replicon chromosome
Accession NZ_CP015150
Organism Bacillus thuringiensis strain Bc601

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BtBc_RS01345 Protein ID WP_000635965.1
Coordinates 239463..239813 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BtBc_RS01340 Protein ID WP_000004570.1
Coordinates 239171..239458 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BtBc_RS01315 234488..235450 + 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -
BtBc_RS01320 235443..236015 - 573 WP_000908522.1 rhomboid family intramembrane serine protease -
BtBc_RS01325 236109..236468 + 360 WP_000583417.1 holo-ACP synthase -
BtBc_RS01330 236625..237575 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
BtBc_RS01335 237693..238862 + 1170 WP_000390616.1 alanine racemase -
BtBc_RS01340 239171..239458 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BtBc_RS01345 239463..239813 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BtBc_RS01350 239881..242049 + 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
BtBc_RS31155 242108..242224 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BtBc_RS01355 242420..242878 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T62513 WP_000635965.1 NZ_CP015150:239463-239813 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T62513 NZ_CP015150:239463-239813 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT62513 WP_000004570.1 NZ_CP015150:239171-239458 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT62513 NZ_CP015150:239171-239458 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References