Detailed information of TA system TA062500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 66757..67358
• Replicon: plasmid pEC732_2
• Accession: NZ_CP015140
• Organism: Escherichia coli strain Ecol_732

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: A4X18_RS26485
• Protein ID: WP_001216034.1
• Coordinates: 66757..67137 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: A4X18_RS26490
• Protein ID: WP_001190712.1
• Coordinates: 67137..67358 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4X18_RS26455	61808..62740	-	933	WP_000081352.1	homocysteine S-methyltransferase	-
A4X18_RS26460	62727..64130	-	1404	WP_001373486.1	S-methylmethionine permease	-
A4X18_RS26465	64374..65354	+	981	WP_000019407.1	IS5-like element IS5 family transposase	-
A4X18_RS26470	65582..65899	+	318	WP_001513659.1	hypothetical protein	-
A4X18_RS29370	65849..66262	-	414	Protein_77	DDE-type integrase/transposase/recombinase	-
A4X18_RS26475	66186..66545	-	360	WP_001513660.1	hypothetical protein	-
A4X18_RS26480	66573..66752	-	180	WP_001513661.1	hypothetical protein	-
A4X18_RS26485	66757..67137	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
A4X18_RS26490	67137..67358	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A4X18_RS26495	67541..69097	+	1557	WP_001617892.1	type I restriction-modification system subunit M	-
A4X18_RS30965	69094..69255	+	162	Protein_83	restriction endonuclease subunit S	-
A4X18_RS26505	69311..70008	+	698	Protein_84	IS1 family transposase	-
A4X18_RS26510	70145..71077	-	933	WP_000991832.1	hypothetical protein	-
A4X18_RS26515	71081..72076	-	996	WP_000246636.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-27 / tet(A)	senB	1..129154	129154
	-	inside	IScluster/Tn	-	-	60851..70008	9157

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T62500 WP_001216034.1 NZ_CP015140:c67137-66757 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T62500 NZ_CP015140:c67137-66757 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT62500 WP_001190712.1 NZ_CP015140:c67358-67137 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT62500 NZ_CP015140:c67358-67137 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6