Detailed information of TA system TA006249

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2402193..2402722
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: parE
• Uniprot ID: P9WHG5
• Locus tag: Rv2142c
• Protein ID: NP_216658.1
• Coordinates: 2402193..2402510 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ75
• Locus tag: Rv2142A
• Protein ID: YP_007410825.1
• Coordinates: 2402507..2402722 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv2138	2397330..2398406	+	1077	NP_216654.1	lipoprotein LppL	-
Rv2139	2398720..2399793	+	1074	NP_216655.1	dihydroorotate dehydrogenase	-
Rv2140c	2399798..2400328	-	531	NP_216656.1	hypothetical protein	-
Rv2141c	2400376..2401722	-	1347	YP_177864.1	hypothetical protein	-
Rv2142c	2402193..2402510	-	318	NP_216658.1	toxin ParE2	Toxin
Rv2142A	2402507..2402722	-	216	YP_007410825.1	antitoxin ParD2	Antitoxin
Rv2143	2402977..2404035	+	1059	NP_216659.1	hypothetical protein	-
Rv2144c	2404165..2404521	-	357	NP_216660.1	transmembrane protein	-
Rv2145c	2404616..2405398	-	783	NP_216661.1	cell wall synthesis protein Wag31	-
Rv2146c	2405666..2405956	-	291	NP_216662.1	transmembrane protein	-
Rv2147c	2406118..2406843	-	726	NP_216663.1	cell division protein SepF	-
Rv2148c	2406840..2407616	-	777	NP_216664.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T6249 NP_216658.1 NC_000962:c2402510-2402193 [Mycobacterium tuberculosis H37Rv]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T6249 NC_000962:c2402510-2402193 [Mycobacterium tuberculosis H37Rv]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT6249 YP_007410825.1 NC_000962:c2402722-2402507 [Mycobacterium tuberculosis H37Rv]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT6249 NC_000962:c2402722-2402507 [Mycobacterium tuberculosis H37Rv]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: P9WHG5

Antitoxin

• AlphaFold DB: P9WJ75

References

(1) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]

(2) Manish Gupta et al. (2016) The Chromosomal parDE2 Toxin-Antitoxin System of Mycobacterium tuberculosis H37Rv: Genetic and Functional Characterization. Frontiers in Microbiology 7:886. [PubMed:27379032]


 experimental literature