Detailed information of TA system TA062483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 3162475..3163900
• Replicon: chromosome
• Accession: NZ_CP015138
• Organism: Escherichia coli strain Ecol_732

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: A4X18_RS15615
• Protein ID: WP_024190590.1
• Coordinates: 3162475..3163545 (+)
• Length: 357 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: E6BSF0
• Locus tag: A4X18_RS15620
• Protein ID: WP_000461705.1
• Coordinates: 3163538..3163900 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4X18_RS28555	3158092..3161490	+	3399	WP_071938245.1	prophage tail fiber N-terminal domain-containing protein	-
A4X18_RS15605	3161490..3162074	+	585	WP_063112476.1	tail fiber assembly protein	-
A4X18_RS28560	3162260..3162415	+	156	WP_001597763.1	hypothetical protein	-
A4X18_RS15615	3162475..3163545	+	1071	WP_024190590.1	type II toxin-antitoxin system RnlA family toxin	Toxin
A4X18_RS15620	3163538..3163900	+	363	WP_000461705.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
A4X18_RS15625	3164038..3164307	-	270	WP_000429347.1	hypothetical protein	-
A4X18_RS15635	3165185..3165667	-	483	WP_000162574.1	SsrA-binding protein SmpB	-
A4X18_RS15640	3165838..3166275	+	438	WP_001339419.1	type II toxin-antitoxin system toxin RatA	-
A4X18_RS15645	3166265..3166555	+	291	WP_001117834.1	RnfH family protein	-
A4X18_RS15650	3166617..3166958	-	342	WP_001203437.1	outer membrane protein assembly factor BamE	-
A4X18_RS15655	3167107..3168768	-	1662	WP_000880939.1	DNA repair protein RecN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (15-112)

Sequences

Toxin

Protein

• Length: 357 a.a.
• Molecular weight: 40475.40 Da
• Isoelectric Point: 7.2332

>T62483 WP_024190590.1 NZ_CP015138:3162475-3163545 [Escherichia coli]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANRNL
GKELADNLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGPYFDKIGSEFILKQQFGRNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Nucleotide

• Length: 1071 bp

>T62483 NZ_CP015138:3162475-3163545 [Escherichia coli]
ATGACAGTCAGGAACTACACTAATTTAAATTTAGATCGGACAACTATTGAGGCTACGTCTAGGGCATTTATTGAAAATAA
AAACTATTCCGTCCATTCAATTGGACCAATGCCTGGTGCAAGAGCAGGACTTCGGGTTGTGTTTGCTAAACCAGGAGAAG
CTTTGGCAACTGTTAACATTTTCTATAATAACGGAGGGACGTCTACAGTCCAGTATCAAACAGGAGCTAATCGTAATCTT
GGAAAAGAGTTGGCTGATAATCTCTATGAAACCATCAATCCTGCAGAATTTGAACAGGTAAATATGGTGTTACAAGGGTT
CGTTGAAGCGAACGTTTTATCTGTACTTCAGCTCTCAGCAGAGCAGCCACATATACAATTTTACGAATACTTACGGAACA
CTCATACAACGGTGTGGAAAATTCATTCGCCAGAATTTCAGGATGAGTTGACAGTTAGTCTGCATCACAGAAATGGAACA
CTACAAATACAAGGGCGTCCATTGTCATGTTATAGAGTGTTTATATTCAACTTGTCAGAGCTACTCGATTTACAAGGACT
GGAAAAAGTTCTCATTCGTCAGGACGATGGCAAAGCGTATATAGTTCAGAAAGAAGTTGCTCGCTCACATCTAGAGTGTG
AAATGGGGGATGCGTACCCACTATTGCATAAGAATGTTGAAAAATTGCTCGTTTCTGGGCTGTGTGTTAAACTCGCCGCC
CCCGATTTACCCGATTACTGTATGTTGTTGTATCCTGAATTACGTTCAGTTGAGGGAGTACTTAAATCAACAATGTACCG
ATATGGCATGCCAATCACGCAGGATGGTTTCGGGCCTTATTTTGATAAAATTGGGAGTGAATTTATTCTGAAACAACAAT
TTGGACGCAATTTATCGTCAGCTAAGGTTAAGACCATTAATGATGCCTATACTTTCTTTAACAGAGAACGTCATGGCCTG
TTTCATATGGAGATAGTTGTCGACACAAGCCGTATGGTTTCTGACATGGCACGACTGATGACTAAGGCCAGACAGGCATG
GGGAATTATTAAGGATTTGTATATTGTTTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13304.23 Da
• Isoelectric Point: 4.2386

>AT62483 WP_000461705.1 NZ_CP015138:3163538-3163900 [Escherichia coli]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Nucleotide

• Length: 363 bp

>AT62483 NZ_CP015138:3163538-3163900 [Escherichia coli]
TTGTTTGAAATAACAGGTGTTAACGTCTCAGGGGCTCTCATTGCCGTCGTTATGGCGACTGGCTACGAGAACCCCTTGAC
TTCAGTTCACGAAATCGAGAAGAGGCTTTCTGTTATACTGGGCAATTCACAGGCTACAGGTGAGGTTTTGTTTGACCTTT
TGTGTTCTAATGGTCCAGAGTGGAATCGCTTTGTTTCGCTGGAAATAAGGTGTGGCAGTATTTTGCTGGATACTGCCAAA
ATTGTGGATGAGCAAGAAATTCCAGCGCAGATCTTGAGACATCAGAGAGATGTATTACGTAACCACCCTGAGTACGTAGA
AGACTCTGTGTTAACACCAGATGATGTTCTTTGTATTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1X9TDS3