Detailed information of TA system TA062478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2152436..2153236
• Replicon: chromosome
• Accession: NZ_CP015138
• Organism: Escherichia coli strain Ecol_732

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: A4X18_RS10460
• Protein ID: WP_000342452.1
• Coordinates: 2152709..2153236 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: A4X18_RS10455
• Protein ID: WP_001277107.1
• Coordinates: 2152436..2152702 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4X18_RS10435	2148095..2148763	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
A4X18_RS10440	2148756..2149814	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
A4X18_RS10445	2150059..2150913	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
A4X18_RS10450	2151184..2152287	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
A4X18_RS10455	2152436..2152702	+	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
A4X18_RS10460	2152709..2153236	+	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
A4X18_RS10465	2153233..2153616	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
A4X18_RS10470	2154039..2155148	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
A4X18_RS10475	2155196..2156122	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
A4X18_RS10480	2156119..2157396	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
A4X18_RS10485	2157393..2158160	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T62478 WP_000342452.1 NZ_CP015138:2152709-2153236 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T62478 NZ_CP015138:2152709-2153236 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT62478 WP_001277107.1 NZ_CP015138:2152436-2152702 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT62478 NZ_CP015138:2152436-2152702 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57