Detailed information of TA system TA062474

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1125304..1126139
• Replicon: chromosome
• Accession: NZ_CP015138
• Organism: Escherichia coli strain Ecol_732

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: A4X18_RS05535
• Protein ID: WP_000854759.1
• Coordinates: 1125304..1125681 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: A4X18_RS05540
• Protein ID: WP_001295723.1
• Coordinates: 1125771..1126139 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4X18_RS30460	1121241..1121417	-	177	Protein_1055	helix-turn-helix domain-containing protein	-
A4X18_RS05515	1122058..1123599	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
A4X18_RS05520	1123614..1124360	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
A4X18_RS05525	1124626..1124802	-	177	WP_000839286.1	DUF957 domain-containing protein	-
A4X18_RS05530	1124819..1125307	-	489	WP_000761690.1	hypothetical protein	-
A4X18_RS05535	1125304..1125681	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
A4X18_RS05540	1125771..1126139	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A4X18_RS05550	1126302..1126523	-	222	WP_000692345.1	DUF987 domain-containing protein	-
A4X18_RS05555	1126586..1127062	-	477	WP_001186775.1	RadC family protein	-
A4X18_RS05560	1127078..1127551	-	474	WP_001350782.1	antirestriction protein	-
A4X18_RS05565	1127893..1128711	-	819	WP_001234738.1	DUF945 domain-containing protein	-
A4X18_RS05570	1128866..1129024	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE	1100145..1140333	40188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T62474 WP_000854759.1 NZ_CP015138:c1125681-1125304 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T62474 NZ_CP015138:c1125681-1125304 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT62474 WP_001295723.1 NZ_CP015138:c1126139-1125771 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT62474 NZ_CP015138:c1126139-1125771 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52