Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 952101..952621 Replicon chromosome
Accession NZ_CP015138
Organism Escherichia coli strain Ecol_732

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag A4X18_RS04660 Protein ID WP_001160964.1
Coordinates 952101..952385 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag A4X18_RS04665 Protein ID WP_000125566.1
Coordinates 952388..952621 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4X18_RS04635 947746..949032 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
A4X18_RS04640 949032..950021 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
A4X18_RS04645 950018..950839 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
A4X18_RS04650 950842..951219 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
A4X18_RS04655 951226..952074 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
A4X18_RS04660 952101..952385 - 285 WP_001160964.1 CcdB family protein Toxin
A4X18_RS04665 952388..952621 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
A4X18_RS04670 952707..953186 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
A4X18_RS04675 953378..955240 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
A4X18_RS04680 955233..955763 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
A4X18_RS04685 955871..957202 - 1332 WP_001183209.1 MFS transporter -
A4X18_RS04690 957261..957548 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T62472 WP_001160964.1 NZ_CP015138:c952385-952101 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T62472 NZ_CP015138:c952385-952101 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT62472 WP_000125566.1 NZ_CP015138:c952621-952388 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT62472 NZ_CP015138:c952621-952388 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References