Detailed information of TA system TA062454

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2894532..2895210
• Replicon: chromosome
• Accession: NZ_CP015137
• Organism: Dickeya solani IPO 2222

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: A4U42_RS12510
• Protein ID: WP_023637601.1
• Coordinates: 2894779..2895210 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A2K8QSU7
• Locus tag: A4U42_RS12505
• Protein ID: WP_023637600.1
• Coordinates: 2894532..2894798 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4U42_RS12485	2890982..2891632	+	651	WP_022632163.1	LysE family translocator	-
A4U42_RS12490	2891703..2892311	-	609	WP_022632164.1	HD domain-containing protein	-
A4U42_RS12495	2892521..2893171	+	651	WP_022632165.1	hemolysin III family protein	-
A4U42_RS12500	2893245..2894225	-	981	WP_022632166.1	tRNA-modifying protein YgfZ	-
A4U42_RS12505	2894532..2894798	+	267	WP_023637600.1	FAD assembly factor SdhE	Antitoxin
A4U42_RS12510	2894779..2895210	+	432	WP_023637601.1	protein YgfX	Toxin
A4U42_RS12515	2895310..2895828	-	519	WP_022632169.1	flavodoxin FldB	-
A4U42_RS12520	2895957..2897282	-	1326	WP_022632170.1	MHS family MFS transporter	-
A4U42_RS12525	2897545..2898444	+	900	WP_022632171.1	site-specific tyrosine recombinase XerD	-
A4U42_RS12530	2898534..2899241	+	708	WP_022632172.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 16808.51 Da
• Isoelectric Point: 11.7737

>T62454 WP_023637601.1 NZ_CP015137:2894779-2895210 [Dickeya solani IPO 2222]
VAQWQCDLRVSWRMQLFSLLTHGFLVLMILLAPWPDGYAPLWLGLVTLVVFGFVRSQRIIKSRQGEISLHGENQLHWQQR
DWQIARRPWVMRNGVLLSLRATTGKGHQRLWLASDSMGNEEWRLLRQLLQQHPLQGTESSRHH

Nucleotide

• Length: 432 bp

>T62454 NZ_CP015137:2894779-2895210 [Dickeya solani IPO 2222]
GTGGCCCAGTGGCAATGTGATTTACGCGTATCCTGGCGCATGCAGTTGTTTTCGCTGCTGACGCACGGGTTTCTGGTGCT
GATGATCCTGCTGGCGCCCTGGCCGGACGGCTATGCGCCGCTGTGGCTGGGGTTGGTGACGCTGGTGGTGTTCGGTTTCG
TGCGCAGTCAGCGCATCATTAAGTCGCGTCAGGGGGAAATTTCCCTGCATGGGGAAAACCAACTGCACTGGCAGCAGCGT
GACTGGCAGATAGCCCGGCGACCGTGGGTGATGCGCAACGGCGTGTTGCTGTCGTTGCGGGCGACGACTGGCAAAGGGCA
TCAGCGTCTGTGGCTGGCGTCAGACAGTATGGGCAATGAAGAGTGGCGGCTGTTGCGCCAGCTGTTGCAGCAGCACCCGC
TGCAGGGAACGGAATCTTCCCGCCATCATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10544.03 Da
• Isoelectric Point: 5.5535

>AT62454 WP_023637600.1 NZ_CP015137:2894532-2894798 [Dickeya solani IPO 2222]
MDINNKSRIHWACRRGMRELDISIMPFFEHEYDTLSDDDKQHFIRLLQCDDPDLFNWLMNHGEPVDPELKRMVSLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT62454 NZ_CP015137:2894532-2894798 [Dickeya solani IPO 2222]
ATGGACATCAATAACAAATCACGTATTCACTGGGCGTGCAGACGCGGAATGCGTGAACTGGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTGAGCGACGACGACAAGCAGCATTTCATCCGTCTGTTGCAGTGCGACGATCCCGACT
TGTTCAACTGGCTGATGAACCATGGCGAGCCGGTGGATCCGGAGCTGAAACGTATGGTTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCAATGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8QSU7