Detailed information of TA system TA062452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2752880..2753664
• Replicon: chromosome
• Accession: NZ_CP015137
• Organism: Dickeya solani IPO 2222

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: A4U42_RS11835
• Protein ID: WP_022632047.1
• Coordinates: 2753170..2753664 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2K8W1V4
• Locus tag: A4U42_RS11830
• Protein ID: WP_022632046.1
• Coordinates: 2752880..2753173 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4U42_RS11810	2748706..2749158	+	453	WP_022632042.1	hypothetical protein	-
A4U42_RS11815	2749201..2749497	+	297	WP_022632043.1	hypothetical protein	-
A4U42_RS11820	2749525..2750232	+	708	WP_022632044.1	SDR family oxidoreductase	-
A4U42_RS11825	2751224..2752475	-	1252	Protein_2320	tyrosine-type recombinase/integrase	-
A4U42_RS11830	2752880..2753173	+	294	WP_022632046.1	DUF1778 domain-containing protein	Antitoxin
A4U42_RS11835	2753170..2753664	+	495	WP_022632047.1	GNAT family N-acetyltransferase	Toxin
A4U42_RS11840	2753716..2754113	+	398	Protein_2323	DUF3577 domain-containing protein	-
A4U42_RS11845	2754229..2755500	+	1272	WP_022632048.1	hypothetical protein	-
A4U42_RS11850	2755504..2756082	+	579	WP_023637591.1	hypothetical protein	-
A4U42_RS11855	2756070..2758010	+	1941	WP_022632050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2752637..2762968	10331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17639.41 Da
• Isoelectric Point: 6.8524

>T62452 WP_022632047.1 NZ_CP015137:2753170-2753664 [Dickeya solani IPO 2222]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSRVLAYYSLASSAVATTAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMILMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T62452 NZ_CP015137:2753170-2753664 [Dickeya solani IPO 2222]
ATGATCTCTGCTCCTGAGCCACTCCACGCCGAACATGTACTATCTTCATTCTGTTGCGGTGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGAACTTTCGTCAGTTGCGATGATTCAAGAGTGTTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACAACTGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAGGGCGTTGGCCGGGCGCTGGTGCGTGATGC
CGGGCTACGGGTGATTCAGGTAGCGGATACTATCGGCATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAAGCGCGTG
ATTTTTACCTACGAGTGGGGTTTGAACCTTCACCGATTGACCCGATGATTTTGATGGTAACGTTGGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10942.53 Da
• Isoelectric Point: 9.8078

>AT62452 WP_022632046.1 NZ_CP015137:2752880-2753173 [Dickeya solani IPO 2222]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT62452 NZ_CP015137:2752880-2753173 [Dickeya solani IPO 2222]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGATCTGATCGA
TCGTGCAGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTTCTTGAGGCTGCTCGCGCCGCCGCAGAGGAAGCGTTGA
TCGATCAGCGTATCATCATGGCAGATCCAGATGCCTATCAGGAATTCCTTGCTCGCTTGGATCAGGCTCCTGAGCCAAAT
GCCGCGCTGCGTAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W1V4