Detailed information of TA system TA062443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 22202..22962
• Replicon: plasmid p35657-1
• Accession: NZ_CP015135
• Organism: Klebsiella pneumoniae strain ATCC 35657

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: WM93_RS27055
• Protein ID: WP_064177585.1
• Coordinates: 22456..22962 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: WM93_RS27050
• Protein ID: WP_003026799.1
• Coordinates: 22202..22468 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM93_RS27000	17887..17985	-	99	WP_004118688.1	type II toxin-antitoxin system RelE/ParE family toxin	-
WM93_RS27005	17972..18151	-	180	WP_065520248.1	type II toxin-antitoxin system ParD family antitoxin	-
WM93_RS27010	18464..18709	+	246	WP_064177598.1	hypothetical protein	-
WM93_RS27015	18714..19286	+	573	WP_064177597.1	hypothetical protein	-
WM93_RS27020	19317..19811	+	495	WP_064177596.1	hypothetical protein	-
WM93_RS27025	19872..20075	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
WM93_RS27030	20089..20319	+	231	WP_049118762.1	hypothetical protein	-
WM93_RS27045	21772..22026	+	255	WP_044246670.1	hypothetical protein	-
WM93_RS27050	22202..22468	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
WM93_RS27055	22456..22962	+	507	WP_064177585.1	GNAT family N-acetyltransferase	Toxin
WM93_RS27060	23145..24491	+	1347	WP_077255522.1	ISNCY family transposase	-
WM93_RS27065	24540..24938	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
WM93_RS27070	25118..26149	+	1032	WP_074194396.1	IS630-like element ISEc33 family transposase	-
WM93_RS27075	26241..26516	-	276	WP_071717249.1	IS3 family transposase	-
WM93_RS27080	26626..27562	+	937	Protein_31	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..158741	158741
	-	inside	IScluster/Tn	-	-	15140..27583	12443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (74-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18119.99 Da
• Isoelectric Point: 9.6090

>T62443 WP_064177585.1 NZ_CP015135:22456-22962 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLRQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
RLIIGVGK

Nucleotide

• Length: 507 bp

>T62443 NZ_CP015135:22456-22962 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCACCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTTCTCGA
TGAATGGTTAAGACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACCG
AAGAAGCTAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTATTCCTTAAGCTTCCA
AGACTAATTATTGGTGTGGGAAAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT62443 WP_003026799.1 NZ_CP015135:22202-22468 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT62443 NZ_CP015135:22202-22468 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TCAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66