Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 172358..173094 Replicon plasmid pKPN-d90
Accession NZ_CP015132
Organism Klebsiella pneumoniae strain Kpn555

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag WM92_RS28125 Protein ID WP_003026803.1
Coordinates 172612..173094 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag WM92_RS28120 Protein ID WP_003026799.1
Coordinates 172358..172624 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM92_RS28075 168420..168782 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
WM92_RS28080 168832..169182 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
WM92_RS28085 169540..169809 + 270 WP_004152102.1 hypothetical protein -
WM92_RS28090 169797..170372 + 576 WP_004152103.1 hypothetical protein -
WM92_RS28095 170403..170897 + 495 WP_004152104.1 DNA-binding protein -
WM92_RS28100 170941..171309 + 369 WP_004152105.1 hypothetical protein -
WM92_RS28105 171343..171546 + 204 WP_004152106.1 hemolysin expression modulator Hha -
WM92_RS28110 171595..171852 + 258 WP_004152107.1 hypothetical protein -
WM92_RS28115 171928..172182 + 255 WP_004152108.1 hypothetical protein -
WM92_RS28120 172358..172624 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
WM92_RS28125 172612..173094 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
WM92_RS28130 173306..174652 + 1347 WP_020314316.1 ISNCY family transposase -
WM92_RS31285 175153..176407 - 1255 Protein_188 IS3 family transposase -
WM92_RS28145 176495..177457 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-1 - 1..224457 224457


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T62425 WP_003026803.1 NZ_CP015132:172612-173094 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T62425 NZ_CP015132:172612-173094 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT62425 WP_003026799.1 NZ_CP015132:172358-172624 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT62425 NZ_CP015132:172358-172624 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References