Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48940..49465 Replicon plasmid pKPN-7c3
Accession NZ_CP015131
Organism Klebsiella pneumoniae strain Kpn555

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag WM92_RS26720 Protein ID WP_001159871.1
Coordinates 48940..49245 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag WM92_RS26725 Protein ID WP_000813634.1
Coordinates 49247..49465 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM92_RS26685 44367..45164 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
WM92_RS26690 45226..45477 + 252 Protein_62 DUF4113 domain-containing protein -
WM92_RS26695 45482..45874 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
WM92_RS26700 45879..46850 - 972 WP_001103694.1 hypothetical protein -
WM92_RS26705 47079..47723 + 645 WP_000633911.1 AAA family ATPase -
WM92_RS26710 47717..47992 + 276 WP_000239529.1 hypothetical protein -
WM92_RS26715 48130..48939 - 810 WP_000016979.1 site-specific integrase -
WM92_RS26720 48940..49245 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
WM92_RS26725 49247..49465 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
WM92_RS26730 51787..52428 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / mph(A) senB 1..142858 142858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T62424 WP_001159871.1 NZ_CP015131:c49245-48940 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T62424 NZ_CP015131:c49245-48940 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT62424 WP_000813634.1 NZ_CP015131:c49465-49247 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT62424 NZ_CP015131:c49465-49247 [Klebsiella pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References