Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4518388..4519007 Replicon chromosome
Accession NZ_CP015130
Organism Klebsiella pneumoniae strain Kpn555

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag WM92_RS22545 Protein ID WP_002892050.1
Coordinates 4518388..4518606 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag WM92_RS22550 Protein ID WP_002892066.1
Coordinates 4518633..4519007 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM92_RS22510 4514434..4514697 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
WM92_RS22515 4514697..4514837 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
WM92_RS22520 4514834..4515532 - 699 WP_004177238.1 GNAT family N-acetyltransferase -
WM92_RS22525 4515633..4517084 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
WM92_RS22530 4517059..4517529 - 471 WP_002892026.1 YlaC family protein -
WM92_RS31760 4517550..4517691 + 142 Protein_4334 hypothetical protein -
WM92_RS22540 4517663..4518229 - 567 WP_002892030.1 maltose O-acetyltransferase -
WM92_RS22545 4518388..4518606 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
WM92_RS22550 4518633..4519007 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
WM92_RS22555 4519493..4522639 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
WM92_RS22560 4522662..4523855 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T62422 WP_002892050.1 NZ_CP015130:c4518606-4518388 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T62422 NZ_CP015130:c4518606-4518388 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT62422 WP_002892066.1 NZ_CP015130:c4519007-4518633 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT62422 NZ_CP015130:c4519007-4518633 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References